IgG (rabbit) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Rabbit IgG Detection Kit is designed for detection and quantitation of rabbit IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

• No-wash steps, no separation steps
• TR-FRET technology
• Sensitive detection
• High reproducibility
• Faster time-to-results
• Easy automation
• 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

The glycoprotein immunoglobulin G (IgG), a major effector molecule of the humoral immune response in rabbit, accounts for the majority of the total immunoglobulins in plasma whereas IgM, IgA, and IgE, each of which has characteristic properties and functions, constitute the remainder The basic IgG molecule has a four-chain structure, comprising two identical heavy (H) chains and two identical light (L) chains, linked together by interchain disulfide bonds.. The present kit recognizes total IgGs Fc fragment.