High-throughput 1536 Well Plate Target Discovery Laboratory Automation Solution

Configuration Requirements:

• Low volume dispensing and compound transfer for 1536-well plate applications
• Versatile system configuration for biochemical, cell-based and cell imaging applications
• Two smaller workstations operate in parallel to increase efficiency and reduce cross contamination between compound reformatting and assay screening aspects of the workflow
• High throughput industrial robot automation
• Enclosed system with HEPA filtration

This configuration that was developed to accommodate two independent process workstation modules linked with a turntable mechanism to produce a fully capable integrated screening system designed to automate the entire screening process. With an integrated 2 x 500 plate storage capacity, the compound workstation module has access to 384,000 compounds in 384-well plate format. Precision dispensing was critical for all aspects of the workflow. Compounds are aliquoted (nL amounts) into the assay plate using a Labcyte^® ECHO^® acoustic dispenser, while reagent and cells are transferred with a Gyger^® Certus^™ instrument (for nL dispensing) and Thermo Scientific^™ Multidrop^™ Combi instruments (for μL dispensing). Assay results are detected using either an EnVision^® Multilabel Plate Reader or an Opera^® Phenix^® confocal microplate imaging reader.