Stable recombinant PhotoScreen® cell line expressing the CRAC / SOCE channel.
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This product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
PhotoScreen (Photina®) photoproteins are a kinetic luminescence technology for measuring calcium flux in cells. It is an alternative technology to fluorescent dyes being used on the FLIPR for studying GPCR targets. The large robust signal generated lets you screen for agonists, antagonists and allosteric modulators with reduced false positives.
We provide two vials of cryopreserved cells (approximately 2.5 x 106 cells/vial) with detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant channel in functional assays (electrophysiology - whole cell voltage clamp and calcium flux luminescence assay).
All cell lines are tested for the absence of mycoplasma.
The main therapeutic interest around CRAC originates from its role in lymphocytes and mast cells, CRAC activation being a requirement for lymphocyte activation. CRAC is currently a research target for allergic disorders, autoimmune and inflammatory diseases. As Store-Operated Calcium Entry (SOCE) is a function found in all higher eukaryotic cells there are many potential therapeutic applications for CRAC regulators.
Terms and conditions apply. Some of our channels may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
| Assay Target Class | Ion channel |
|---|---|
| Assay Target Type | Cell line |
| Assay Validated | Calcium Luminescence |
| Host Cell | HEK293 |
| Product Brand Name | PhotoScreen |
| Quantity in a Package Amount | 5.0 Million Cells |
| Receptor Type | CRAC |
| Second Messenger Release | Calcium flux |
| Shipping Condition | Dry Ice |
| Unit Size | 2 vials |
Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance
Ca2+-activated photoproteins are important tools for analyzing all aspects of Ca2+-mediated signal transduction processes in mammalian cells. One of their characteristics is the immediate photon release (flash luminescence) upon Ca2+ binding to the coelenterazine photoprotein complex, which makes this system extremely useful for studying rapid receptor-ligand interactions or fast acting ion channels involving Ca2+ mobilization.
We have shown here that signal intensity for the selected catalog aequorin cell lines is strong enough to allow its measurement by the non-luminescence dedicated FLIPR
