PerkinElmer
check quantity

Harmony High-Content Imaging and Analysis Software

Easily quantify complex cellular phenotypes with Harmony® high-content analysis software. Harmony software is designed for PerkinElmer high-content screening systems.

Part Number
Version Number
Product Compatibility
HH17000010
4.9
Operetta CLS AND Opera Phenix
more
HH17000001
4.8
Operetta
more
HH17000012
5.0
Opera Phenix Plus
more
Buy Now

Please enter valid quantity

Please log in to add favorites.

NULL OR EMPTY CART

Overview

Designed for biologists, Harmony’s workflow-based interface makes the whole process of high-content analysis straightforward, even for new users with little microscopy or programming knowledge.

One high-content analysis software for all your applications

  • Phenotypic Screening
    • Get robust phenotypic fingerprints based on intensity, morphology, texture and fluorescence distribution parameters
    • Easily train the software to recognize relevant phenotypes
    • Quantify even subtle phenotypic differences
  • Live Cell Imaging
    • Follow phenotypic changes over time
    • Accurately quantify label-free live cell images
    • Track individual cells over time or analyze wound-healing assays
    • Analyze cell signaling dynamics using e.g. FRET based assays
  • 3D Cell Models
    • Speed up 3D image acquisition by targeted imaging of microtissues
    • Better understand your cell models by exploring them in the new 3D viewer and the XYZ viewer
    • Measure morphologies and volumes in 3D, count nuclei within spheroids
    • Enhance Maximum Intensity Projection Analysis using PlaneMap
    • One software for image acquisition, 3D visualization and analysis
  • Rare Cell Phenotypes
    • Quickly capture your rare cell phenotypes with PreciScan
    • Pre-screen at low resolution and acquire objects of interest at high resolution
    • Shorten your image acquisition times
    • Reduce your data volume
  • Routine Assays
    • Utilize more than 30 ready-made-solutions for standard assays
    • Ensure repeatability by running established protocols

One high-content analysis software for all users

  • New Users
    • Get quantification for your cell images
    • Easy to learn – even for users with little microscopy or programming knowledge
    • Get started with 30 ready-made solutions
    • Logical workflow for easy control of all aspects of image acquisition and analysis
    • Simple building blocks to assemble image analysis sequences (step-by-step)
  • Expert Users
    • Quantify difficult phenotypes with advanced texture and morphology readouts
    • Utilize advanced building blocks to enable e.g. ratiometric FRET imaging
    • Create “stitched” global images from multiple fields of view
    • Examine samples at multiple scales – regions found in the global image can be used for analysis in the original images
  • For Lab Managers
    • Keep up with increasing research needs – analyze larger sample sizes
    • Quickly train new users to set up the instrument and analyze their images
    • Find images, metadata and results quickly via the integrated sortable database
    • View and analyze data from any computer with installation of Harmony software
    • Get application support by image analysis experts

Specifications

Product Compatibility Operetta CLS AND Opera Phenix
Version Number 4.9
Resources, Events & More
  • All

Application Note

A Multiparametric Live-Cell Cytotoxicity Analysis using the Operetta

The detection of compound cytotoxicity is an essential part of drug discovery. In this work we describe a rapid and flexible image-based live cell approach to study cytotoxicity. Based on the use of a fluorophore dye mixture, we analyzed multiple cellular phenotypic changes following a toxic insu ...

PDF 1 MB
Analysis of Mitochondrial Dynamics in Human iPSC-derived Neurons using the Operetta CLS High-Content Analysis System

Mitochondrial dynamics are essential for energy conversion and neuron survival. Understanding changes in mitochondrial dynamics is therefore crucial in developing mitochondria-based therapy options for complex pathological conditions such as cancer, neurological disorders, and metabolic syndromes ...

PDF 821 KB
Imaging Bile Canaliculi in 3D Liver Microtissues

Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. A functional impairment of hepatobilary transporters, such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP-2), is strongly associated wit ...

PDF 2 MB
Label-Free Analysis of Cardiomyocyte Beating using the Opera Phenix Plus System

Drug-induced cardiotoxicity is a frequent cause of drug attrition during drug development and can lead to the withdrawal of drugs from the market, so non-invasive approaches that allow quick and reliable assessment of the cardiotoxic potential of a compound are key to improve drug safety.

I ...

PDF 1 MB
Measuring FRET using the Opera Phenix High-Content Screening System

Fundamental processes in living cells, such as apoptosis and signal transduction are controlled by proteins, often acting in concert with other protein partners through protein-protein interactions (PPIs). Inappropriate protein-protein recognition can fundamentally contribute to many diseases, inclu ...

PDF 1 MB

Article

Cell Painting: Your Questions Answered

With powerful potential for phenotypic drug discovery, cell painting is being rapidly adopted by leading scientists across the globe. However, implementing cell painting assays can present some challenges.

To help you avoid some of the common pitfalls, we’ve collated answers from our expert ...

PDF 470 KB

Brochure

3D Cell Culture Solutions Brochure

Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cells.

PDF 4 MB
Operetta CLS High-Content Analysis System Brochure

The product of more than a decade of experience in high-content screening, the Operetta CLS delivers the speed and sensitivity for everyday assays, and even complex challenges – live cells, phenotyping, rare events, and much more.

PDF 2 MB

Ebook

High-Content Analysis: Advancing Your Knowledge to Help Combat Infectious Disease

Infectious diseases remain a major burden to human health. The increased globalization of modern society that facilitates the spread of infectious diseases, and phenomena such as anti-microbial resistance, underscore the importance of the development of new preventative and therapeutic approaches ...

PDF 3 MB

Technical Note

How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System

Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. Learn how you can perform successful five-day live cell imaging on Operetta CLS™ and Opera Phenix™ high-content systems, avoid phototoxicity with gentle digital phase cont ...

PDF 3 MB
Opera Phenix High-Content Screening System Technical Performance: Speed

The Opera Phenix™ high-content screening system’s state of the art hardware, combined with Harmony® imaging and analysis software, improves acquisition and analysis speed, enabling users to perform highly multi-parametric phenotypic screens effectively. In this technical note, learn how the Opera P ...

PDF 708 KB

White Paper

Artificial Intelligence, Machine Learning and Deep Learning: Applications in Cellular Imaging for Improved Drug Discovery Productivity

There has been a lot of buzz around artificial intelligence, machine learning and deep learning. Is the reality living up to the hype?

In the world of cellular imaging and its application to drug discovery, there is evidence of real progress against some of the critical challenges facing s ...

PDF 1 MB
High Content Screening in Three Dimensions

Researchers are increasingly looking to 3D cell cultures, microtissues, and organoids to bridge the gap between 2D cell cultures and in vivo animal models. This whitepaper documents a streamlined procedure for getting the most information, as quickly as possible, using solutions from PerkinElmer.

PDF 1 MB