GreenSep™ Ethyl Pyridine is an endcapped phase and has proven superior to conventional stationary phases (such as diol, cyano etc.) in the areas of separation selectivity, peak shape and loading capacity. The chromatogram shown (right) is a prime example of the superior peak shape performance obtainable with the GreenSep Ethyl Pyridine column with SFC. Mobile phase composition and fraction collection is greatly simplified without the use of amino additives. GreenSep Ethyl Pyridine provides better separation for amines in comparison with the GreenSep Ethyl Pyridine II.
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Many SFC separations have utilized “older normal phase HPLC types” of stationary phases such as unmodified silica, diol, amino and cyano. These phases are poorly adapted to SFC and present a number of limitations for SFC separations including low capacity, poor selectivity and poor peak shape. GreenSep stationary phases, on the other hand, have been specifically engineered for SFC separations, paying close attention to bonding coverage, density and all factors leading to high capacity phases which exhibit excellent selectivity and peak shape. Many of the GreenSep phases designed for basic and acidic compounds do NOT require mobile phase additives that are commonly required with other brands of phases. The GreenSep range features a variety of selectivities offering orthogonality. All of these materials are available in analytical and also semi-preparative (10 mm), and preparative (20 mm, 30 mm and 50 mm i.d.) dimensions. Additionally, comprehensive technical and method development assistance is offered. Shown below is a column selection guide for the development of a SFC separation with the GreenSep Pyridyl Amide being the go-to column of choice, followed by the other columns.
Features and Benefits:
|Inner Diameter||30 mm|
|Particle Size||5.0 µm|