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GrowDex®-T 10 ml

GrowDex®-T is an animal-free ready to use hydrogel that has been specifically developed to offer superior imaging properties, but without compromising on any of the advantages of the original hydrogel GrowDex. GrowDex-T can be mixed directly with cells and culture media, no gelation or cross-linking step required. Ambient handling and shear thinning properties of GrowDex-T enable easy dispensing and use in automated systems.

GrowDex-T is for research use only, not for use in diagnostic or therapeutic purposes.

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GrowDex-T is a transparent negatively charged hydrogel that consists of nanofibrillar cellulose (1.0% w/v) and 99.0% purified water and is supplied ready to use. The transparent nature of GrowDex-T offers superior imaging properties, makes it ideal for use in brightfield, phase contrast and confocal microscopy applications as well as high content imaging. GrowDex-T is ready to use and can be mixed directly with cells and culture media, no gelation or cross-linking step required. Ambient temperature handling and shear thinning properties of GrowDex-T enable easy handling and use in automated dispensing systems. The product is supplied sterilized.


  • Animal-free: No animal biomolecules interfering with readouts.
  • Lot to lot reproducibility: nanofibrillar cellulose and purified water only. GrowDex hydrogel is manufactured to the highest standards with strict quality control criteria.
  • Reliable: robust and sustained performance regardless of the production lot ordered.
  • Stable: used and stored at room temperature, GrowDex hydrogel can be used in liquid handling systems.
  • Ready-to-use: simplified protocol in which GrowDex hydrogel is mixed with media and cells.
  • Tunable: Optimal gel stiffness is achieved by dilution with cell culture media. Matrix composition can be optimized easily by supplementing cell culture media with specific biomolecules such as growth factors.
  • Compatibility with High Content Screening systems: no auto-fluorescence and transparency.

Storage and shelf-life:

  • Storage: Ambient
  • Shelf life: 12 months
  • Shelf life after opening: 3 months


  • GrowDex-T is supplied at a concentration of 1.0%.
  • Working concentrations are usually between 0.2% and 0.8%.


Shipping Condition Ambient
Unit Size 10mL
Resources, Events & More
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Application Note

Diffusion rates for small drug molecules in GrowDex-T hydrogel

In this study diffusion rates of small drug molecules, namely nadolol, ketoprofen and metronidazole, through GrowDex-T were evaluated.

PDF 214 KB
GrowDex-T Supports hiPSC-derived Neural Progenitor Differentiation in vitro

Three-dimensional in vitro culture of neuronal cells is necessary to recapitulate the complex cytoarchitecture of the central nervous system. There is a need for defined substrates that can allow for the differentiation of iPSC-derived neurons and astrocytes. Here we establish methodologies for 3D c ...

PDF 285 KB
Osteosarcoma cell line MG-63 viability in GrowDex-T

In this study the osteosarcoma cell line MG-63 was used to find a working method for spheroid formation in GrowDex®-T hydrogel. Analysis was done using different cell stains and with high content confocal microscopy.

PDF 970 KB
Recommended Procedure For Diluting and Mixing GrowDex-T

GrowDex-T can easily be diluted with e.g. cell culture media, PBS, or ultra-pure water for use in cell-based assays. The optimal concentration will depend on the cell type being used. This application note provides a recommended procedure for the dilution and mixing of Growdex-T.

PDF 368 KB

Data Sheet

GrowDex-T Instructions for Use

This document provides instructions for use, safety information, storage instructions, ordering and contact information for GrowDex-T.

PDF 115 KB


GrowDex Technical Protocols

This guide describes a number of experimental protocols performed using GrowDex hydrogels.

PDF 928 KB
Guide to Reducing the Cost and Time to Set-up Reproducible HTS/HCS with 3D Cell Models

Download our guide to reducing the costs and time required to set up reproducible high-throughput and high-content screens using 3D cell culture models.

Topics include:

  • Opportunities and challenges in 3D cell culture models for HTS and HCS
  • Challenges of scaling up 3D HTS/ ...

PDF 616 KB
How To Transfer From Animal-Derived Matrices to GrowDex - 3D Cell Culture Matrix

Animal-derived matrices for 3D cell culture are often poorly defined and suffer from lot-to-lot variability. This variability ultimately affects the reproducibility of the experiment, which is an essential element for reliable research studies.

Natural, reliable and ready-to-use, GrowDex