GrowDex®-T is an animal-free ready to use hydrogel that has been specifically developed to offer superior imaging properties, but without compromising on any of the advantages of the original hydrogel GrowDex. GrowDex-T can be mixed directly with cells and culture media, no gelation or cross-linking step required. Ambient handling and shear thinning properties of GrowDex-T enable easy dispensing and use in automated systems.
GrowDex-T is for research use only, not for use in diagnostic or therapeutic purposes.
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GrowDex-T is a transparent negatively charged hydrogel that consists of nanofibrillar cellulose (1.0% w/v) and 99.0% purified water and is supplied ready to use. The transparent nature of GrowDex-T offers superior imaging properties, makes it ideal for use in brightfield, phase contrast and confocal microscopy applications as well as high content imaging. GrowDex-T is ready to use and can be mixed directly with cells and culture media, no gelation or cross-linking step required. Ambient temperature handling and shear thinning properties of GrowDex-T enable easy handling and use in automated dispensing systems. The product is supplied sterilized.
Storage and shelf-life:
In this study diffusion rates of small drug molecules, namely nadolol, ketoprofen and metronidazole, through GrowDex-T were evaluated.
Three-dimensional in vitro culture of neuronal cells is necessary to recapitulate the complex cytoarchitecture of the central nervous system. There is a need for defined substrates that can allow for the differentiation of iPSC-derived neurons and astrocytes. Here we establish methodologies for 3D c ...
In this study the osteosarcoma cell line MG-63 was used to find a working method for spheroid formation in GrowDex®-T hydrogel. Analysis was done using different cell stains and with high content confocal microscopy.
GrowDex-T can easily be diluted with e.g. cell culture media, PBS, or ultra-pure water for use in cell-based assays. The optimal concentration will depend on the cell type being used. This application note provides a recommended procedure for the dilution and mixing of Growdex-T.
This document provides instructions for use, safety information, storage instructions, ordering and contact information for GrowDex-T.
This guide describes a number of experimental protocols performed using GrowDex hydrogels.
Download our guide to reducing the costs and time required to set up reproducible high-throughput and high-content screens using 3D cell culture models.
Animal-derived matrices for 3D cell culture are often poorly defined and suffer from lot-to-lot variability. This variability ultimately affects the reproducibility of the experiment, which is an essential element for reliable research studies.
Natural, reliable and ready-to-use, GrowDex