PerkinElmer
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GrowDex® 2.5 ml

GrowDex® is an animal-free hydrogel that enables complete control over the culture environment for cells.

GrowDex can be used in a wide range of cell-based assays such as microscopic imaging or metabolic activity measurement. GrowDex has been validated on various cell models and applications including primary hepatocytes, patient derived cells, 3D organoid formation, hepatocyte toxicity, stem cell proliferation and differentiation, tumour cell migration and invasion, neurite outgrowth and network formation.

GrowDex is for research use only and should not be used for diagnostic or therapeutic purposes.

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100103002
2.5mL
155.00 USD
 
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100103005
5mL
205.00 USD
 
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100103010
10mL
373.00 USD
 
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Overview

GrowDex has been designed to provide a support matrix for the culture of cells in a wide range of applications. It comprises of two components, nanofibrillar cellulose (NFC) and ultra-pure water. The length of the nanofibrils ranges from 200-300 μm, with a diameter in the nanometer scale varying from 5-100 nm.

The product is supplied sterilized, ready to use and can be utilized in a variety of cell culture applications, such as 3D spheroid or organoid formation.

Benefits:

  • Animal-free: No animal biomolecules interfering with readouts.
  • Lot to lot reproducibility: nanofibrillar cellulose and purified water only. GrowDex hydrogel is manufactured to the highest standards with strict quality control criteria.
  • Reliable: robust and sustained performance regardless of the production lot ordered.
  • Stable: used and stored at room temperature, GrowDex hydrogel can be used in liquid handling systems.
  • Ready-to-use: simplified protocol in which GrowDex hydrogel is mixed with media and cells.
  • Tunable: Optimal gel stiffness is achieved by dilution with cell culture media. Matrix composition can be optimized easily by supplementing cell culture media with specific biomolecules such as growth factors.
  • Compatibility with High Content Screening systems: no auto-fluorescence and transparency.

Storage and shelf-life:

  • Storage: Ambient
  • Shelf life: 12 months
  • Shelf life after opening: 3 months

Concentration

  • GrowDex is supplied at a concentration of 1.5%.
  • Working concentrations are usually between 0.2% and 1.0%.

GrowDase enzyme breaks down GrowDex hydrogel to form a solution in an easy one step efficient cell recovery process. 3D cell structures such as spheroids, organoid or biopsies are retained with no impact on cell viability or functionality. Liberated cells can be used in gene or protein expression studies or for future experiments.

Specifications

Shipping Condition Ambient
Unit Size 2.5mL
Resources, Events & More
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Application Note

3D In vitro culture of the neuronal cell line SH-SY5Y is sustained in GrowDex

In this application note, we establish methodologies for 3D cell culture of the common neuronal cell line SH-SY5Y within GrowDex hydrogel to provide a more biomimetic set of environmental cues with which to promote cell migration and neurite outgrowth, whilst sustaining the viability of the populati ...

PDF 300 KB
Adipose derived mesenchymal stem cells: Investigating sphere formation in relatiin to seeding density and hydrogel concentration

Notably, mesenchymal stem cells (MSCs) are the most researched cells for their potential use in cell-based therapies. As such, it is crucial to fully characterise these cells within novel 3D environments prior to their use and application for such therapies. In this application note, adipose derived ...

PDF 546 KB
Breast Cancer Cell Line MCF7 3D Culture Demonstrated in GrowDex and Matrigel

"MCF7 is an adenocarcinoma cell line derived from breast tissue with metastatic and pleural effusion morphology. As it is one of the few cancer cell lines expressing estrogen receptor a (ER-a), it is the most published cell line in the field of breast cancer research. In this study, the morphology d ...

PDF 486 KB
GrowDex Supports Robust Human Neuronal Network Formation in vitro

In this study pre-differentiated human pluripotent stem cell derived neurons were encapsulated within GrowDex and cultured.

PDF 532 KB
GrowDex supports hiPSC-derived neural progenitor differentiation in vitro

In this study, we establish methodologies for 3D cell culture of human iPSC-derived neural progenitors within nanofibrillar cellulose hydrogel, GrowDex®.

PDF 344 KB
Guidelines for using GrowDex in cell-based assays

GrowDex can easily be diluted with e.g. cell culture media, PBS, or ultra-pure water for use in cell-based assays. The optimal concentration will depend on the cell type being used. This application note provides a recommended procedure for the dilution and mixing of Growdex.

PDF 226 KB
High Throughput Cytotoxicity Testing with HepG2 Cells Grown in 3D Culture

In this application note, we address some of the challenges of producing 3D culture models, using GrowDex® hydrogel as a 3D cell culture matrix for growing liver-derived HepG2 cells in 384-well microtiter plates.

PDF 596 KB
MCF7 3D Culture in GrowDex and Matrigel

In this study, the morphology development of MCF7 cells in GrowDex® and in Matrigel is presented.

PDF 486 KB
Novel NRAS mutated human melanoma cell line MUG-Mel2 3D culture in GrowDex

In this application note, 3D culture of a novel NRAS mutated human melanoma cell line MUG-Mel2 in GrowDex is presented.

PDF 373 KB

Data Sheet

GrowDex Instructions for Use

This document provides instructions for use, safety information, storage instructions, ordering and contact information for GrowDex.

PDF 118 KB

Guide

GrowDex Technical Protocols

This guide describes a number of experimental protocols performed using GrowDex hydrogels.

PDF 928 KB
Guide to Reducing the Cost and Time to Set-up Reproducible HTS/HCS with 3D Cell Models

Download our guide to reducing the costs and time required to set up reproducible high-throughput and high-content screens using 3D cell culture models.

Topics include:

  • Opportunities and challenges in 3D cell culture models for HTS and HCS
  • Challenges of scaling up 3D HTS/ ...

PDF 616 KB
How To Transfer From Animal-Derived Matrices to GrowDex - 3D Cell Culture Matrix

Animal-derived matrices for 3D cell culture are often poorly defined and suffer from lot-to-lot variability. This variability ultimately affects the reproducibility of the experiment, which is an essential element for reliable research studies.

Natural, reliable and ready-to-use, GrowDex

PDF 2 MB