GrowDase™ enzyme breaks down GrowDex® hydrogel to form a solution in an easy one- step efficient cell recovery process. 3D cell structures such as spheroids, organoid or biopsies are retained with no impact on cell viability or functionality. Liberated cells can be used in gene or protein expression studies or for future experiments.
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GrowDase is a purified mixture of cellulase enzymes that has been specifically developed to break down the nano-cellulose fibrils present in GrowDex into soluble glucose. The process is simple, enzyme is mixed with the sample and incubated at 37°C until the GrowDex has been fully reduced to glucose. Enzymatic removal allows cells to be recovered from the matrix efficiently, retain their 3D structure, e.g., spheroid or organoid, intact and without adverse affect on cell functionality. The amount of GrowDase required for cell recovery is dependent on the amount of GrowDex (cellulose) present in the sample. The product is supplied sterilized.
Storage and shelf-life:
This document provides instructions for use, safety information, storage instructions, ordering and contact information for GrowDase.
This guide describes a number of experimental protocols performed using GrowDex hydrogels.