Fluorescently-labeled Fluorescein-12-ddUTP nucleotide analog for enzymatic incorporation, for direct fluorescence detection.
Fluorescein labeled dideoxyuridine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenaseâ„¢. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations.
Concentration |
1.0 mm
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Volume |
50.0 µL
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Buffer |
10 mM Tris-HCl, pH 7.6, 1 mM EDTA
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Detection Method |
Direct
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Fluorophore |
Fluorescein
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Nucleotide |
ddUTP
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Shipping Conditions |
Shipped Ambient
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Unit Size |
50 nmol
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