PerkinElmer
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Fluorescein-12-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.

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NEL465001EA
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Overview

This fluorescein labeled analog of adenosine triphosphate (ATP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. Fluorescein labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. Labeled products may be detected directly via fluorescence or indirectly by combining with anti-fluorescein HRP or AP conjugates.

The number “12” in the compound name refers to the length of the linker arm between ATP and the label.

Specifications

buffer 10 mM Tris-HCl, pH 7.6, 1 mM EDTA
Concentration 1 mM
Detection Method Fluorescence
Fluorophore Fluorescein
Nucleotide dATP
Shipping Condition Ambient
Unit Size 25 nmol