Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
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This fluorescein labeled analog of adenosine triphosphate (ATP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. Fluorescein labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. Labeled products may be detected directly via fluorescence or indirectly by combining with anti-fluorescein HRP or AP conjugates.
The number “12” in the compound name refers to the length of the linker arm between ATP and the label.
|buffer||10 mM Tris-HCl, pH 7.6, 1 mM EDTA|
|Unit Size||25 nmol|
Fluorescent nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerizes. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling, or transcription of RNA using SP6, T3, or T7 RNA polymerases may be used.