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Flexar UHPLC

For demanding applications when you need to collect more information in less time, our Flexar UHPLC is the perfect solution. This UHPLC is designed for a high productivity environment and delivers high sensitivity and resolution, exceptional flow accuracy and precision and faster results.

Part Number N2910535
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When you need the ultimate performance for your LC analysis, look no further than the Flexar UHPLC. This ultra- high performance UHPLC system delivers the highest resolution, highest sensitivity and fastest analysis. Across food, consumer product, pharmaceutical, industrial and environmental applications, the Flexar UHPLC performs reliably and with the flexibility to perform quality testing of raw materials, determine fraud/adulteration of products, ensure lot to lot consistency, as well research-based analysis for new products. Our Flexar UHPLC is engineered for labs that need more uptime, less maintenance and the ability to handle multiple methods – and its low carry over design enables quantitatively more accurate and repeatable results. Additional flexibility includes the choice of our powerfully easy-to-use Chromera® chromatography data system (CDS) software or TotalChrom® for for either single workstation or enterprise-wide control and regulatory compliance.

Our Flexar UHPLC is configured to provide the highest flow rate up to 5mL/min. with a maximum of 18K psi, a high speed autosampler for fast injection rates, choice of ovens and detectors, including the PDA Plus, which offers the highest acquisition rates in the industry.


Model Name FX-20 Pump


System Components
Description Part Number


FX-20 Pump




UHPLC Autosampler




Standard Column Oven

Peltier Column Oven





FX-UV/Vis Detector

PDA Plus Detector




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Application Note

A Robust Method for the Analysis of Commonly Used Sunscreen Compounds for Compliance with New FDA Regulations

Over time, exposure to ultra violet (UV) ,radiation from the sun or tanning beds can ,damage the skin’s cellular DNA, resulting in ,mutations that cause 3.5 million cases of skin ,cancers and about 11,500 deaths in the U.S. ,each year, for a total cost of nearly $2 billion. ,There are three types of UV: UVC, UVB, and ,UVA.

Analysis of Biocides with the PerkinElmer Flexar FX-15 UHPLC System Equipped with a PDA Detector

Biocides are chemical substances that are used to kill or inhibit harmful organisms. Biocides have a wide range of applications in consumer and industrial products.

PDF 955 KB
Analysis of Chlorophenols in Water by UHPLC with PDA Detection

This application will focus on the solid phase extraction (SPE) and HPLC analysis of chlorophenols in three tap water samples.

PDF 989 KB
Analysis of Color Additives in Beverages with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector

Dyes are used to intensify the color of food products and make them look tempting. They are also used to minimize color variation, and to prolong color stability on shelf. This application note presents a fast and robust HPLC method for the determination of dyes in beverages. Method conditions and performance data including precision and linearity are presented. A popular orange soda is analyzed and the type and amount of dyes used are confirmed.

PDF 924 KB
Analysis of Common Antioxidants in Edible Oil with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector

This note describes a number of important food applications utilising the PerkinElmer DSC demonstrating the versatility of the technique as a tool in the food industry.

Analysis of Drug Substances in Common Cold Medicines with the PerkinElmer Flexar FX-15 System Equipp

The misuse of cold remedies can be prevented by appropriate medical indications and accurate label claims. To that end, the U.S. Food and Drug Administration and the pharmaceutical industry have made it a standard procedure to routinely test drug products to ensure the accuracy of the amount of active ingredients. This application note presents a method for the simultaneous analysis of acetaminophen, dextromethorphan and phenylephrine. Method conditions and precision are presented. A cold medicine tablet is analyzed and the type and amount of active ingredient are confirmed.

PDF 823 KB
Analysis of Fat-Soluble Vitamins by UHPLC Using UV Detection

In 2009, the United States Pharmacopeial Convention introduced the USP Dietary Supplements Compendium (DSC) – an industry directed resource featuring regulatory guidance, documents, supplemental information, and reference tools.

Analysis of Ginsenosides in Ginseng Root with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector

Ginseng has been used as an herbal medicine in Asia for over two thousand years for its purported various health benefits, including antioxidant, anticarcinogenic, antiinflammatory, antihypertensive and anti-diabetic. The pharmacologically active compounds behind the claims of ginseng’s efficacy are ginsenosides; their underlying mechanism of action although not entirely elucidated appears to be similar to that of steroid hormones. There are a number of ginseng species, and each has its own set of ginsenosides.

PDF 511 KB
Analysis of Isoflavones in Soy Products by UHPLC with UV Detection

A great variety of isoflavone supplements and soy products, some additionally fortified, are available on the market today. Although a number of analytical methods for isoflavones exist, the goal of this work was to develop a simpler, faster and reliable LC method for the analysis of the six most widely-used isoflavones in soy products.

Analysis of Isoflavones with the PerkinElmer Flexar FX-15 UHPLC System Equipped with a PDA Detector

Because of the potential health benefit of isoflavones, many soy products and isoflavone supplements are available to consumers. This application note presents a fast, robust, and sensitive reverse phase liquid chromatography method for the analysis of six widely used isoflavones. Method conditions and performance data including precision and linearity are presented. Two soy dietary supplement tablets are analyzed and the isoflavones type and amount are confirmed.

PDF 992 KB
Analysis of Patulin in Apple Juice by UHPLC with UV Detection

In food, organic acids, such as malic, ascorbic and citric acids, originate from natural biochemical processes or are added as preservatives, acidulants and/or stabilizers. Organic acids contribute to the sensory properties of foods and beverages by providing both taste and aroma. In particular, citric acid is widely used in soft drinks to provide the sour citrus taste. The monitoring of these organic acids is essential for quality control during the processing of juices and related products, as well as for evaluating juice authenticity and purity. In addition, the use of organic acids in foods and beverages is regulated in many countries, though regulations vary widely. This application note presents a simple and robust HPLC method for the analysis of organic acids typically found in store-bought fruit juices.

Analysis of Vanillin, Ethyl Vanillin, and Coumarin in Vanilla Extract Products by UHPLC with PDA Detection

Honey consumption has grown significantly during the last few decades due to its high nutritional value and unique flavor. The price of natural bee honey is much higher than,other sweeteners making it susceptible to adulteration with cheaper sweeteners, primarily sucrose. Besides lower levels of nonsugar ingredients, natural honey primarily consists of glucose and fructose and may contain low levels of sucrose and/or maltose. However, according to the international regulations, any commercially available “pure”-labeled honey products that are found to have in excess of 5% by weight of sucrose or maltose are considered to be adulterated. With the focus on possible honey adulteration, this application highlights the LC separation of various sugars found in honey and the analysis of these components in four storebought honey samples. Method conditions and performance data, including linearity and repeatability, are presented.

Analysis of the Mycotoxin Patulin in Apple Juice Using the Flexar FX-15 UHPLC-UV

Patulin is produced by various molds, which primarily infect the moldy part of apples. Removing the moldy and damaged parts of the fruit may not eliminate all the patulin because some of it may migrate into sound parts of the flesh.

PDF 913 KB
Cannabinoid Monitoring in Dried Cannabis Flowers

This application describes an analytical method for the chromatographic separation and quantitative monitoring of seven primary cannabinoids, including THC and THC-A, in cannabis extracts by HPLC with PDA detection. Naturally occurring cannabinoids, the main biologically active component of the cannabis plant, form a complex group of closely related compounds, of which 113 are known and 70 are well described. Of these, the primary focus has been on ?9-tetrahydrocannabinol (THC), as the primary active ingredient due to its pharmacological and toxicological characteristics, upon which strict legal limits have been enforced.

Cannabinoid Monitoring in a Variety of Edibles

Butylated hydroxytoluene (BHT) is a common food additive used to protect foods from spoilage. Concern exists that long-term human consumption of BHT may have potential health risks. Analysis of BHT is needed for both food quality and safety reasons.

Determination of a-acids in Hops and Beers

The use of cannabis concentrates and CBD products (edibles, topicals, vape products, etc.) has increased in popularity as new regulation has been passed legalizing medicinal and recreational use in a number of states and provinces. Testing to ensure that pesticide and mycotoxin levels in cannabis products are below regulatory levels is critical, however, the cannabis concentrate matrix presents many analytical challenges including higher sample matrix effects and an increased concentration of cannabinoids in the sample.

In this application note, an LC/MS/MS method for the analysis of 66 pesticides (including hydrophobic and chlorinated pesticides typically analyzed by GC/MS/MS) and five mycotoxins in a cannabis concentrate matrix is presented. Utilizing a QSight® 420 LC/MS/MS instrument with dual APCI and ESI sources, the analysis yielded excellent recoveries and detection limits below those specified by the state of California cannabis regulations.

PDF 906 KB
Fast Analysis of Fat-Soluble Vitamins Using Flexar FX-10 and Chromera CDS

Sweeteners are low or zero-calorie sugar substitutes that are added in drinks, processed foods and pharmaceutical products to provide the sweet taste of table sugar, which is also called sucrose.

PAHs in Surface Water by PDA and Fluorescence Detection Application Note

Heightened awareness of polycyclic aromatic hydrocarbons (PAHs) has become prevalent due to urban background levels found in surface water, soil, air, cosmetics and food. They are generated by the combustion of fossil fuels and are always found as a mixture of individual compounds that differ in behavior, environmental distribution, and their effect on biological systems. PAHs encompass a wide molecular weight range, differing based on their physical, chemical, and biological characteristics. PAHs in surface water result from a variety of sources including residential, industrial and commercial outlets, streets and parking lots, and atmospheric fallout. In this application, via a spiking experiment, we explore the levels at which PAHs in surface water can be monitored by UHPLC with a sub-2 µm particle sized column combined with photo diode array (PDA) and fluorescence (FL) detection.,

Polymer Applications Compendium

Today’s plastics are some of the most used materials on a global volume basis. Broadly integrated into today’s industrial and commercial lifestyles, they make a major, irreplaceable contribution to virtually every product category.

In this compendium you will find a wide range of applications for polymers, plastics, rubbers and advanced materials. Discover how to put these applications to work for you simply and efficiently.

Quality Control of Drug Substances in Headache Medicines with PerkinElmer Flexar UHPLC System Equipped with a PDA Detector

Acetaminophen and aspirin are the drugs of choice used to relieve the symptoms of common headache. Acetaminophen, which is also called paracetamol, is widely used as a pain reliever (analgesic) and fever reducer (antipyretic). Because of its fast onset (eleven minutes after intake), acetaminophen is very effective. However, every year its misuse (dose exceeding the daily adult limit of four grams) can cause fatal liver damage. This application note presents a modified USP method for the analysis of aspirin, acetaminophen and caffeine using a superficially porous particle column and a PerkinElmer® UHPLC platform.

Quantification of Benzodiazepines in Plasma Using UHPLC Time-of-flight Mass Spectrometry

Presented is an alternative technique to quantitate benzodiazepines in plasma using a rapid protein precipitation method with a fast LC separation method in combination with TOF-MS.

Rapid UHPLC Determination of Common Preservatives in Cosmetic Products

This application note will present a fast, sensitive and reliable UHPLC analysis of six common parabens. A PerkinElmer Flexar FX-15 UHPLC system fitted with a Flexar FX PDA photodiode array detector was used. The separation was achieved using a PerkinElmer Brownlee Analytical C18, 1.9 µm 50 mm x 2.1 mm column.

PDF 960 KB
Rapid UHPLC Determination of Nine Common Herbicides in Drinking Water with the PerkinElmer Flexar FX

Water polluted by herbicides leach and runoff can cause human health problems including cancer tumors, reproduction deformity, disruption of the endocrine system and DNA damage. This application presents a sensitive and robust liquid chromatography method to test nine widely used herbicides (Figure 1), using a 3 µm UHPLC column to achieve very high throughput at a low flow rate to reduce testing time and solvent consumption. The throughput is compared to that of a conventional C18 HPLC column. Method conditions and performance data including precision and linearity, are presented.

PDF 750 KB
Simultaneous Analysis of Nine Food Additives with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector

Food additives are natural or synthetic substances that are added in food, beverage and pharmaceutical products for their microbicidal, preservative and flavoring properties. This application note presents a fast and robust liquid chromatography method to simultaneously test nine widely used additives. Among the additives tested are: preservatives (benzoic acid, sorbic acid, dehydroacetic acid and methylparaben); artificial sweeteners (acesulfame potassium, saccharin and aspartame); flavoring agent (quinine); and a stimulant (caffeine).

PDF 938 KB
The Analysis of Sunscreen-Active Ingredients and Parabens in Lotions and Lip Balms by UHPLC with PDA Detection

Individuals typically use 5-20 cosmetics per day, many of which contain sunscreen to prevent skin damage from the sun’s radiation, and antimicrobial preservatives called parabens. Although sunscreen-active ingredients are designed to block UV radiation, some cell damage may be caused when these ingredients are illuminated by sunlight after absorption into the skin. For example, oxybenzone, an ingredient considered safe by the FDA (Food and Drug Administration), is believed to contribute to the recent rise in melanoma cases by increasing the production of DNA-attacking free radicals upon UV exposure. Additionally, studies have shown oxybenzone to behave similarly to the hormone estrogen, suggesting that it may also contribute to the development of breast cancer. Parabens are absorbed through the skin via cosmetic applications and can be found in nearly all adult urine samples, with the highest concentrations observed in adult females and adolescents. Furthermore, parabens are thought to have estrogenic activity, which affects the expression of genes regulated by the natural form of estrogen, leading to early puberty in girls and an increased risk for the development of breast cancer.

PDF 890 KB
The Qualitative and Quantitative Analysis of a-Acids in Hops and Beers by UHPLC with UV Detection

Alpha acids (a-acids) are a class of chemical compounds of primary importance in the production of beer. They are found in the resin glands of the flowers of the hop plant (Humulus lupulus) and are normally added to the boil after mashing the grains, providing beers with their aroma and bitter taste. The a-acids found in hop resins are isomerized to form the iso-a-acids during prolonged boiling in the wort. The degree of isomerization and the amount of bitter taste produced by the addition of hops is highly dependent on the type of hop and the length of time the hops are boiled. Longer boil times will result in isomerization of more of the available a-acids, making the beer more bitter. The a-acid percentages vary within specific varieties of hops, depending on the growing conditions, drying methods, age of hops, climate and other factors. Figure 1 shows the common a-acids and iso-a-acids involved in the beer brewing process. Since the quality and quantity of a-acids is so important in consistently providing individual beers with their recognizable taste, it is essential to monitor their amount in hops and beers and to monitor the formation of the iso-a-acids during the beer brewing process. The focus of this application note is to provide an easy, straightforward, and robust analytical method for establishing the type and amount of a-acids in hops pellets, as well as determining the amount of a-acids and iso-a-acids in various beers.



QSight Triple Quadrupole LC/MS/MS

Whether you’re a lab manager or a bench chemist, you know firsthand how things are changing quickly in food and environmental analysis. And your lab needs to change to keep pace.



Advanced Solutions for Polymers and Plastics Poster

The Polymer Market consists of a huge diversity of manufacturers of industrial products running many different processes yet still facing similar challenges. There is more and more pressure to achieve high product quality and reduce costs in order to stay one step ahead of the competition.


White Paper

Assessment of Extractables and Leachables Associated with Pharmaceutical Packaging

Extractables and leachables studies are critical for maintaining the quality of your drug product and complying with GMP regulations during drug development and final batch release. Exposure to extractables and leachables could have a detrimental impact on the safety and efficacy of a final drug product. Testing for these contaminants is critical in every part of pharmaceutical packaging including the packaging system used to store drug products.

PDF 881 KB
UHPLC Instrument and Method Transfer Guidelines Whitepaper

Over the past few years, there has been tremendous interest in approaches to speed up and/or increase the resolving power of the analytical separation process, particularly with the development of columns packed with porous sub-2 µm particles used in very high pressure conditions. Many laboratories want to transition some of their conventional HPLC methods to fast UHPLC methods, but their lack of experience some¬times acts as a deterrent to trying.

In this white paper, we share our experience in HPLC-to-UHPLC method transfer in the form of a tutorial introductory guide to help those who want to try this new and exciting UHPLC methodology to improve their productivity.