DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and antibody-dependent cell cytotoxicity (ADCC). This kit has the same assay principle as a chromium release assay, with target cells loaded with a BATDA ligand instead of 51Cr. There is no requirement for specially-engineered cells.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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DELFIA time-resolved fluorescence cell cytotoxicity assays offer a non-radioactive method to Chromium 51, for use in cell-mediated cytotoxicity and antibody-dependent cell cytotoxicity (ADCC) studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.
Reagents for the assay are also available separately:
|Detection Method||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA|
|Shipping Condition||Blue Ice|
|Unit Size||1 Kit|
Discover PerkinElmer's simplified, gentle cell-labelling method for non-radioactive cytotoxicity assays
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This product is intended for loading of cells to be used in short term cytotoxicity tests performed utilizing time-resolved fluorometry in the detection.