PerkinElmer
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DELFIA Eu-N1 Streptavidin

DELFIA® Europium-labeled streptavidin, for use in DELFIA time-resolved fluorescence (TRF) assays.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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Overview

DELFI® Eu-labeled streptavidin is intended for use as a generic reagent to detect biotin-labeled compounds, e.g. antibodies or antigens in various types of immunoassay or nucleic acid probes in hybridization assays based on solid-phase separation. The label, Eu3+, is measured using dissociation-enhanced time-resolved fluorometry.

The streptavidin is isolated from fermentation filtrates of Streptomyces avidinii and 1 mg of streptavidin binds approximately 10 μg of biotin. The preparation is supplied ready to use in 50 mmol/L Tris-HCl buffered saline solution. For DELFIA immunoassays, a final concentration of 100 ng/mL is recommended for the generation of a suitable signal.

Specifications

Antibody Conjugates Streptavidin
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), DELFIA TRF
Experimental Type In vitro
Fluorophore Eu-N1
Product Brand Name DELFIA
Shipping Condition Blue Ice
Unit Size 250 µg
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Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.

Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.

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