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LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) Antibody, 100 µg

Europium-labeled monoclonal antibody recognizing human Histone H3 that is unmodified at lysine 9 and lysine 27 (H3K9/K27). For use in LANCE® Ultra epigenetic writer and eraser assays.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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10 µg
4428.00 USD
100 µg
11100.00 USD
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The LANCE® Ultra Europium-anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) antibody was used for the development and optimization of epigenetic writer and eraser assays.


  • 10 µg = 1,500 assay points (384-well assay format)
  • 100 µg = 15,000 assay points (384-well format)

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.

Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.


Antibody Conjugates Anti-H3K9/H3K27
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore Eu-W1024
Format Microplates
Molecular Modification Acetylation,Methylation
Product Brand Name LANCE Ultra
Shipping Condition Dry Ice
Therapeutic Area Oncology
Unit Size 100 µg
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Application Brief

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Technical Note

LANCE Ultra HDAC1 Histone H3-Lysine 9 Deacetylase Assay

In this technical note, we present the optimization of an HDAC1, enzymatic assay using as substrate a biotinylated histone H3-derived, peptide acetylated at lysine 9. The deacetylated peptide product is, captured by the Eu-labeled antibody (Eu-Ab) and ULight-Streptavidin, (ULight-SA), which bring th ...

PDF 678 KB
LANCE Ultra JMJD1A Histone H3-Lysine 9 Demethylase Assay

LANCE Ultra time-resolved fluorescence resonance energy transfer, (TR-FRET) assays use a proprietary europium chelate donor dye, W1024 (Eu), together with ULight™, a small molecular weight, acceptor dye with a red-shifted fluorescent emission.

PDF 734 KB