Rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human MEK1 phosphorylated at Ser217 and Ser221. It also reacts with MEK1 singly phosphorylated at Ser217 or Ser221. MEK1 is phosphorylated in vivo by RAF1 and COT. Homologous phosphorylation sites are found in MEK2. Sequence motif recognized: GQLIDSMANSFVG
true falseFor research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) assays use a proprietary europium chelate donor dye, W1024 (Eu), together with ULight™, a small molecular weight acceptor dye with a red-shifted fluorescent emission. Validated for over 310 kinases, we provide data transparency by supplying:
For this antibody:
In this kinase assay configuration, the phosphorylated biotinylated substrate is detected by both the ULight-labeled streptavidin and Eu-labeled anti-phosphosubstrate antibodies, which brings donor and acceptor dyes into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight substrate phosphorylation.
| Antibody Conjugates | Anti-phospho-MEK1/2 |
|---|---|
| Automation Compatible | Yes |
| Detection Method | Time-Resolved Fluorescence (TRF), TR-FRET |
| Experimental Type | In vitro |
| Fluorophore | Eu-W1024 |
| Molecular Modification | Phosphorylation |
| Product Brand Name | LANCE Ultra |
| Shipping Condition | Blue Ice |
| Unit Size | 10 µg |
Protein kinases play a major role in normal cellular functions such as cell proliferation, angiogenesis, and cell adhesion. Dysregulation of kinase activity has been shown to be associated with several human diseases including cancer, diabetes and morphological disorders. This makes kinases crucial targets for the discovery and development of new drugs.
Dysregulation of kinase activity has been shown to be associated with several human diseases including cancer, diabetes and morphological disorders. Because of their function, kinases are crucial targets for drug discovery. Analysis of the human genomehas revealed the existence ofnearly 520 genes encoding kinases.
LANCE® Ultra is a high through putscreening (HTS) technology platform optimized for homogeneous time-resolved fluorescence resonance energy transfer (TRFRET) kinase assays. The LANCEUltra donor dye is a Europium(Eu) chelate, and the acceptor dye is a proprietary small-molecular weight molecule called ULight™, a fluorescent dye with a red-shifted light emission.
Protein kinases play a major role in normal cellular functions such as cell proliferation, angiogenesis, and cell adhesion. Dysregulation of kinase activity has been shown to be associated with several human diseases including cancer, diabetes and morphological disorders. This makes kinases crucial targets for drug discovery. Analysis of the human genome has revealed the existence of nearly 520 genes encoding kinases.
Protein kinases are involved in the regulation of many aspects of the cellcycle, including proliferation, differentiation, secretion and apoptosis. Aberrant protein kinase expression or functioning is a cause orconsequence of many human diseases. As a result, kinases have become extremely popular for drug discovery programs and this has prompted the development of many kinase assay technologies suitable for highthrough putscreening (HTS).
Protein tyrosine kinases (PTK) are involved in the regulation of many aspects of the cell cycle, including proliferation, differentiation, secretion and apoptosis. Aberrant protein kinase expression or function is a cause or consequence of many human diseases like cancer and atherosclerosis. As a result, kinases became very important targets in drug discovery.
Of the 518 known human kinases, 90 are tyrosine (Tyr) kinases. Protein Tyr kinases play a key role in signal transduction and normal cell growth. They are also involved in numerous proliferative diseases like cancer and atherosclerosis, in addition to a number of autoimmune diseases. For these reasons, Tyr kinases are often targets for new drug discovery in many laboratories involved in basic R&D, disease and therapeutics research, and HTS.
