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EnSight Multimode Plate Reader

The EnSight multimode plate reader offers HTS Alpha, Ultra-sensitive Luminescence and fast well-imaging technologies alongside conventional multimode detection technologies. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.

Part Number HH34000000
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For research use only. Not for use in diagnostic procedures.


The EnSight system's cell-imaging option, brings image-based cytometry together with our industry-leading detection technologies for the first time. Imaging is fast, with online data analysis in minutes.

The system has a modular design that lets you add detection modes as your needs change, and combined with workflow-based Kaleido data acquisition and analysis software, you have a truly versatile plate reader that gets users productive quickly – making it ideal for multiuser environments.

  • Well-imaging technology, Alpha technology and conventional multimode detection technologies all on a single benchtop system
  • Easily configurable and field upgradable
  • Base unit with filter-based absorbance technology and temperature control unit (up to 65°C)
  • Eight barcode identified filter positions
  • Integrated barcode reading from all four sides of a microplate
  • Kaleido Data Acquisition and Analysis Software


  • Fluorescence well-imaging with single cell resolution for up to four colors (with excitation of three colors in parallel), brightfield and digital phase-contrast imaging
  • AlphaLISA® / AlphaScreen® detection with high power laser excitation
  • Fluorescence intensity detection (top- and bottom-reading) with monochromator
  • Time-resolved fluorescence detection, for example DELFIA® and LANCE® technologies
  • Ultra-sensitive luminescence for low signals
  • Filter- and optional monochromator-based absorbance detection

Key Features:

  • Temperature control to 65°C
  • Plate barcode reader
  • Plate shaking
  • Plate stackers, 20 plates or 50 plates
  • Robotic Interface for automation solutions


Automation Compatible Yes
Detection Method Absorbance, Image-based Cytometry, Time-Resolved Fluorescence (TRF), Luminescence, Alpha, Fluorescence
Product Brand Name EnSight
Resources, Events & More
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Application Note

Automated Detection of Germinated Arabidopsis thaliana Seeds in Microplates

In this application note, the imaging module of EnSight® multimode plate reader was used to automatically detect the viability of Arabidopsis thaliana, a model organism often used by plant biologists for different research purposes.

A custom script created for the Kaleido software was used to detect seeds at early stages of the germination process: brightfield and fluorescence images were used to count seeds and to automatically compute the percentage of germinated seeds present in each well. Seed germination was modulated by treating seeds with different concentration of the plant hormone abscisic acid or NaCl (salinity stress). MyAssays® Desktop was used for analysis of the kinetic data, that were automatically acquired every 4 hours by EnSight.

The traditional approach based on visual inspection of germinating seeds is time-consuming and has limitations. But with the automated approach, the EnSight can accelerate the quantification of the germination rate of A. thaliana seeds, generating reproducible and robust results. Read this application note to learn more.

Effects of 5FU and Sorafenib on Proliferation and Biomarker Expression in a Colorectal Cancer Model Using AlphaLISA and EnSight Solutions

A variety of chemotherapeutic drugs with different modes of action have been developed and tested as potential therapies for colorectal cancer. Characterizing the effects of potential drugs with different modes of action is a key part of the process.

In this application note you will learn:

  • How to rapidly measure multiple biomarkers in both cell culture supernatant and lysates from the same wells of a microplate to examine complex protein expression profiles from a colorectal cancer cellular model
  • Benefits of using AlphaLISA® technology for characterizing the effects of potential drugs with different modes of action on a cell culture model of human colorectal cancer
  • How to measure the effects of drug treatments, such as 5FU and Sorafenib, on cellular proliferation by automated well-imaging and cell counting using the EnSight® multimode plate reader
  • Examples of the data and analysis you can generate for such biomarker and proliferation assays



3D Cell Culture Solutions Brochure

Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cells.

Drug Discovery Screening Solutions Brochure

Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automated liquid handling, assays and reagents, imaging and detection systems, and informatics.

Working independently or together, with our solutions you can achieve consistent and accurate results. By accelerating the identification and characterization of effective and safe drug candidates, the PerkinElmer portfolio enables you to optimize efficiency in your lab and deliver more actionable, real-world results.

Download the brochure to learn more about how we can partner with you so that you can discover smarter, more effective, data-driven breakthroughs in the critical screening stages of drug discovery and development.


Field Application Report

A Plate-based Time-resolved Fluorescence Method for Quantifying Carbonylation Damage in Protein Samples Field Application Report

High sample density microplate reader technology combined with high sensitivity, time-resolved fluorescence (TRF) measurement, contribute to research in monitoring and studying the effects of various environmental stressors on Baltic Sea flora

PDF 300 KB
Studying Environmental Stress Levels in Baltic Sea Zooplankton Limnocalanus Macrurus Using Biochemical Markers Finland University of Turku Field Application Report

Report presents a method EnSpire™ Multilabel Plate Reader to detect environmental stress levels in organisms at the base ofthe marine food web. It shows results from 8 L. macrurus samples collected from one study site

PDF 499 KB

Technical Note

Increased Throughput and Reduced Variability of Virus Plaque Assays with Automated Imaging and Analysis

Plaque assays are a standard method utilized by virologists to identify the amount of infectious virus particles in solution. In many laboratories, counting the plaques in plates is performed manually either by visual inspection or using a microscope. This is cumbersome, error prone and very time-consuming. Automated imaging and plaque analysis are rarely used.

In this note, we show how the plaque imaging and analysis process can be automated to help virologists rapidly determine the concentrations of virus solutions with higher precision and less effort using the EnSight® multimode plate reader and Kaleido™ Data Acquisition and Analysis Software. When combined with 96-well microplates and EnSight’s stacker system, this method further enhances the time-saving benefit and qualifies for high-throughput screening (HTS) applications. Download our technical note to learn more.