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EnSight Multimode Plate Reader

The EnSight multimode plate reader offers HTS Alpha, Ultra-sensitive Luminescence and fast well-imaging technologies alongside conventional multimode detection technologies. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.

Part Number HH34000000
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For research use only. Not for use in diagnostic procedures.


The EnSight system's cell-imaging option, brings image-based cytometry together with our industry-leading detection technologies for the first time. Imaging is fast, with online data analysis in minutes.

The system has a modular design that lets you add detection modes as your needs change, and combined with workflow-based Kaleido data acquisition and analysis software, you have a truly versatile plate reader that gets users productive quickly – making it ideal for multiuser environments.

  • Well-imaging technology, Alpha technology and conventional multimode detection technologies all on a single benchtop system
  • Easily configurable and field upgradable
  • Base unit with filter-based absorbance technology and temperature control unit (up to 65°C)
  • Eight barcode identified filter positions
  • Integrated barcode reading from all four sides of a microplate
  • Kaleido Data Acquisition and Analysis Software


  • Fluorescence well-imaging with single cell resolution for up to four colors (with excitation of three colors in parallel), brightfield and digital phase-contrast imaging
  • AlphaLISA® / AlphaScreen® detection with high power laser excitation
  • Fluorescence intensity detection (top- and bottom-reading) with monochromator
  • Time-resolved fluorescence detection, for example DELFIA® and LANCE® technologies
  • Ultra-sensitive luminescence for low signals
  • Filter- and optional monochromator-based absorbance detection

Key Features:

  • Temperature control to 65°C
  • Plate barcode reader
  • Plate shaking
  • Plate stackers, 20 plates or 50 plates
  • Robotic Interface for automation solutions


Automation Compatible Yes
Detection Method Absorbance, Image-based Cytometry, Time-Resolved Fluorescence (TRF), Luminescence, Alpha, Fluorescence
Product Brand Name EnSight
Resources, Events & More
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Field Application Report

A Plate-based Time-resolved Fluorescence Method for Quantifying Carbonylation Damage in Protein Samples Field Application Report

High sample density microplate reader technology combined with high sensitivity, time-resolved fluorescence (TRF) measurement, contribute to research in monitoring and studying the effects of various environmental stressors on Baltic Sea flora

PDF 300 KB
Studying Environmental Stress Levels in Baltic Sea Zooplankton Limnocalanus Macrurus Using Biochemical Markers Finland University of Turku Field Application Report

Report presents a method EnSpire™ Multilabel Plate Reader to detect environmental stress levels in organisms at the base ofthe marine food web. It shows results from 8 L. macrurus samples collected from one study site

PDF 499 KB

Technical Note

Increased Throughput and Reduced Variability of Virus Plaque Assays with Automated Imaging and Analysis

Plaque assays are a standard method utilized by virologists to identify the amount of infectious virus particles in solution. In many laboratories, counting the plaques in plates is performed manually either by visual inspection or using a microscope. This is cumbersome, error prone and very time-consuming. Automated imaging and plaque analysis are rarely used.

In this note, we show how the plaque imaging and analysis process can be automated to help virologists rapidly determine the concentrations of virus solutions with higher precision and less effort using the EnSight® multimode plate reader and Kaleido™ Data Acquisition and Analysis Software. When combined with 96-well microplates and EnSight’s stacker system, this method further enhances the time-saving benefit and qualifies for high-throughput screening (HTS) applications. Download our technical note to learn more.