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EnSight Multimode Plate Reader

The EnSight® multimode plate reader offers HTS Alpha, Ultra-sensitive Luminescence and fast well-imaging technologies alongside conventional multimode detection technologies. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.

Part Number HH34000000
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For research use only. Not for use in diagnostic procedures.


The EnSight system's cell-imaging option, brings image-based cytometry together with our industry-leading detection technologies for the first time. Imaging is fast, with online data analysis in minutes.

The system has a modular design that lets you add detection modes as your needs change, and combined with workflow-based Kaleido data acquisition and analysis software, you have a truly versatile plate reader that gets users productive quickly – making it ideal for multiuser environments.

  • Well-imaging technology, Alpha technology and conventional multimode detection technologies all on a single benchtop system
  • Easily configurable and field upgradable
  • Base unit with filter-based absorbance technology and temperature control unit (up to 65°C)
  • Eight barcode identified filter positions
  • Integrated barcode reading from all four sides of a microplate
  • Kaleido Data Acquisition and Analysis Software

Our Enhanced Security option for the Kaleido software provides technological controls and features that support 21 CFR Part 11 compliance. It empowers users based on their authorization level to assign access, implement data security, approve workflows with electronic signatures, and view all user actions from a comprehensive audit trail.


  • Fluorescence well-imaging with single cell resolution for up to four colors (with excitation of three colors in parallel), brightfield and digital phase-contrast imaging
  • AlphaLISA® / AlphaScreen® detection with high power laser excitation
  • Fluorescence intensity detection (top- and bottom-reading) with monochromator
  • Time-resolved fluorescence detection, for example DELFIA® and LANCE® technologies
  • Ultra-sensitive luminescence for low signals
  • Filter- and optional monochromator-based absorbance detection

Key Features:

  • Temperature control to 65°C
  • Plate barcode reader
  • Plate shaking
  • Plate stackers, 20 plates or 50 plates
  • Robotic Interface for automation solutions

Selected Publications:

  • De novo Neurosteroidogenesis in Human Microglia: Involvement of the 18 kDa Translocator Protein. Germelli L, Da Pozzo E, Giacomelli C, et al. Int J Mol Sci. 2021;22(6):3115. Published 2021 Mar 18.
  • Stable neutralizing antibody levels 6 months after mild and severe COVID-19 episodes. Pradenas E, Trinité B, Urrea V, et al. Med (N Y). 2021;2(3):313-320.e4.
  • Discovery of a First-In-Class Small Molecule Antagonist against the Adrenomedullin-2 Receptor: Structure-Activity Relationships and Optimization. Zirimwabagabo JO, Jailani ABA, Avgoustou P, et al. J Med Chem. 2021;64(6):3299-3319.
  • Stabilizing the closed SARS-CoV-2 spike trimer. Juraszek J, Rutten L, Blokland S, et al. Nat Commun. 2021;12(1):244. Published 2021 Jan 11.
  • Modified mRNA/lipid nanoparticle-based vaccines expressing respiratory syncytial virus F protein variants are immunogenic and protective in rodent models of RSV infection. Espeseth, A.S., Cejas, P.J., Citron, M.P. et al. npj Vaccines 5, 16 (2020).
  • Inhibiting the P2X4 Receptor Suppresses Prostate Cancer Growth In Vitro and In Vivo, Suggesting a Potential Clinical Target. He J, Zhou Y, Arredondo Carrera HM, et al. Cells. 2020;9(11):2511. Published 2020 Nov 20.
  • Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2. Nie J, Li Q, Wu J, et al. Emerg Microbes Infect. 2020;9(1):680-686.

For additional publications, please visit Google Scholar.


Automation Compatible Yes
Detection Method Absorbance, Image-based Cytometry, Time-Resolved Fluorescence (TRF), Luminescence, Alpha, Fluorescence
Product Brand Name EnSight
Resources, Events & More
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Application Note

Automated Detection of Germinated Arabidopsis thaliana Seeds in Microplates

In this application note, the imaging module of EnSight® multimode plate reader was used to automatically detect the viability of Arabidopsis thaliana, a model organism often used by plant biologists for different research purposes.

A custom script created for the Ka ...

Culture and Quality Control of Human iPSC-Derived Cells Using an Automated High Throughput Approach

High throughput screening (HTS) in iPSC (induced pluripotent stem cells)-derived neuronal cell types with the intent to identify novel therapeutic compounds for neurodegenerative diseases has the potential to transform the way drug discovery is performed. But the use of neuronal stem cells for sc ...

Effects of 5FU and Sorafenib on Proliferation and Biomarker Expression in a Colorectal Cancer Model Using AlphaLISA and EnSight Solutions

A variety of chemotherapeutic drugs with different modes of action have been developed and tested as potential therapies for colorectal cancer. Characterizing the effects of potential drugs with different modes of action is a key part of the process.

In this application note you will learn: ...



3D Cell Culture Solutions Brochure

Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cells.

Drug Discovery Screening Solutions Brochure

Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...



Choosing the Right Plate Reader

Selecting the right plate reader for your lab can be challenging. There are multiple factors to consider including: detection type, additional features, support, and price. With numerous options for each variable, it can be confusing to navigate the selection process. Focusing on striking a balan ...

Detection Technologies for Your Scientific Breakthroughs

There are many options available today to help labs with their scientific research. Understanding the latest detection technologies, assay platforms, and microplates can inform you to make better decisions and set your lab up for success. PerkinElmer offers an optimal solution to meet your divers ...

Solving the Complexity of Compliance

Reaching compliance according to the laws and regulations of each country can be a tedious, complicated process, and being GxP compliant (FDA 21 CFR Part 11, EU Annex 11, etc.) is necessary in order to deliver therapeutic treatments to market for pharmaceutical and biotechnology companies, includ ...


Field Application Report

A Plate-based Time-resolved Fluorescence Method for Quantifying Carbonylation Damage in Protein Samples Field Application Report

High sample density microplate reader technology combined with high sensitivity, time-resolved fluorescence (TRF) measurement, contribute to research in monitoring and studying the effects of various environmental stressors on Baltic Sea flora

PDF 300 KB
Studying Environmental Stress Levels in Baltic Sea Zooplankton Limnocalanus Macrurus Using Biochemical Markers Finland University of Turku Field Application Report

Report presents a method EnSpire™ Multilabel Plate Reader to detect environmental stress levels in organisms at the base ofthe marine food web. It shows results from 8 L. macrurus samples collected from one study site

PDF 499 KB

Technical Note

Increased Throughput and Reduced Variability of Virus Plaque Assays with Automated Imaging and Analysis

Plaque assays are a standard method utilized by virologists to identify the amount of infectious virus particles in solution. In many laboratories, counting the plaques in plates is performed manually either by visual inspection or using a microscope. This is cumbersome, error prone and very time ...


White Paper

21 CFR Part 11 Guidance for EnSight Multimode Plate Reader

To achieve compliance with the regulations of the Food and Drug Administration (FDA) as defined in the 21 CFR Part 11 and other references like the EU Annex 11, you need a well aligned combination of software tools and implemented administrative procedures. With the Enhanced Security option for t ...

PDF 961 KB