ELAST® uses tyramide signal amplification (TSA) to improve sensitivity and enable reduced consumption of detection antibodies in HRP-based ELISA.
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The smaller size ELAST kit makes it easier to evaluate signal amplification for your ELISA.
| Detection Method | Radiometric |
|---|---|
| Shipping Condition | Blue Ice |
TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.
The PerkinElmer ELAST® (ELISA Amplification System) is designed to amplify the signal generated by the enzyme horseradish peroxidase (HRP) in solid phase analytical methods such as ELISA. ELAST is based on the use of PerkinElmer’s proprietary Tyramide Signal Amplification (TSA) technology. The ELISA reporter enzyme, HRP, catalyzes the covalent binding of biotinyl-tyramide to the solid phase of assay wells. Subsequent reaction with streptavidin-HRP causes additional HRP to be bound to the solid phase resulting in signal amplification.
The PerkinElmer Life Sciences, Inc. ELAST® (ELISA Amplification system) is designed to amplify the signal generated by the enzyme horseradish peroxidase (HRP) when applied to solid phaseanalytical methods such as the ELISA. The final enhanced signal is primarily due to the catalytic activity of additional HRP deposited on the solid phase
