PerkinElmer
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easylite-Kinase, 100 mL

ATP detection assay for general kinase activity.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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6066746
10 mL
110.00 USD
 
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6066741
100 mL
410.00 USD
 
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Overview

While cell-based assays are increasingly used for kinase screening, biochemical assays remain an advantageous tool in the development of kinase modulators as they provide a method to directly assess the activity of a compound in a pure system. Biochemical assays allow insights into the mechanism of action of candidate compounds that may be important to achieve the desired kinase inhibition characteristics and selectivity.

While one way to identify kinase inhibitors of therapeutic interest is to monitor phosphorylated substrate formation, a quicker and universal approach is to monitor the ATP consumption after the kinase assay reaction. easylite-Kinase™ is a detection system that uses luciferin and a luciferase to produce a luminescent signal in proportion to the amount of ATP remaining after stopping the kinase activity. In the easylite-Kinase system, kinase inhibitors will prevent ATP consumption by the kinase, and the ATP remaining will generate a positive luminescent signal.

Luminescence assays have the advantage of being quick and easy to read without requiring microplate readers with specialized excitation sources or filters. The luminescence signal produced gives a high signal-to-background ratio, which expedites assay development time.

Benefits:

  • Linear from 10 nM to 10 μM ATP
  • Homogeneous
  • Streamlined workflow with a single reagent step
  • Rapid results
  • Small scale or high-throughput
  • Stable reagents
  • Kinase inhibitors lead to increased signal

Specifications

Assay Target Class Kinase
Assay Technology Luminescence
Automation Compatible Yes
Detection Method Luminescence
Experimental Type In vitro
Product Brand Name easylite-Kinase
Shipping Condition Ambient
Unit Size 100 mL