Stable, recombinant AequoScreen® cell line expressing aequorin and the Dopamine D2L receptor, human recombinant in CHO-K1 host cells.
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Cell Line Terms and Conditions must be accepted before orders are placed. Additional agreement needed for commercial use.
PerkinElmer’s AequoScreen® double transfected cell lines are provided in two vials of frozen cells, each containing ∽2.5 x 106 cells. Detailed product information as well as recommended cell culture conditions and validation assay date are available for each cell line.
The AequoScreen technology is a generic GPCR technology that can be used with Gs, Gi, and Gq-coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
PerkinElmer’s AequoScreen cell lines require accepted cell line Terms and Conditions prior to order processing. Additional agreement needed for commercial use. Contact us using the Request More Information button above for additional information regarding required documentation.
|Assay Target Class||GPCR|
|Assay Target Type||Cell line|
|Assay Validated||Calcium Luminescence|
|G-Alpha Natural Receptor||Gi/Go|
|Product Brand Name||AequoScreen|
|Second Messenger Release||Calcium flux|
|Shipping Condition||Dry Ice|
|Special Ordering Information||Requires accepted Terms and Conditions. Additional agreement needed for commercial use.|
|Therapeutic Area||Central Nervous System|
|Unit Size||5 million cells|
Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.
Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute the activea equorin enzyme.
PerkinElmer’s validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. So you can concentrate on results.
Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance
FroZen, -irradiated cells, are a well established product, that can be ordered as a consumable, and readily used to perform Aequorin functional assays (AequoZen) or cAMP assays (cAMPZen). While, for the G s–coupled receptors, an increase of cAMP is detected in such an assay, for G i-coupled receptors an inhibition of the forskolin-stimulated level of, cAMP is detected.