Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
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This dinitrophenol (DNP) labeled analog of uridine triphosphate (UTP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. DNP labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. DNP-labeled products may be detected with anti-DNP antibody conjugates. For the highest sensitivity in detection, we recommend using a anti-DNP-HRP conjugate with tyramide signal amplification (TSA).
The number “11” in the compound name refers to the length of the linker arm between UTP and the label.
|buffer||10 mM Tris-HCl, pH 7.6, 1 mM EDTA|
|Unit Size||25 nmol|
TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.
Nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerases. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling, or transcription of RNA using SP6, T3, or T7 RNA polymerases may be used.