Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
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Dinitrophenol (DNP) labeled deoxyuridine triphosphate (dUTP) is useful for labeling with a variety of methods including nick translation, random priming, polymerase chain reaction (PCR), and 3'-end labeling. DNP-labeled products may be detected with anti-DNP conjugates. For the highest sensitivity in detection, we recommend using anti-DNP-HRP conjugate with tyramide signal amplification (TSA).
The number “11” in the compound name refers to the length of the linker arm between dUTP and the label.
| buffer | 10 mM Tris-HCl, pH 7.6, 1 mM EDTA |
|---|---|
| Concentration | 1 mM |
| Hapten | DNP |
| Nucleotide | dUTP |
| Shipping Condition | Ambient |
| Unit Size | 25 nmol |
TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.
Nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerases. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling, or transcription of RNA using SP6, T3, or T7 RNA polymerases may be used.
