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DirectDetect SARS-CoV-2 Real-time RT-PCR for Environmental Surfaces

The DirectDetect SARS-CoV-2 Real-time RT-PCR kit for Surfaces features the ability to move directly from the sample to PCR using a simplified workflow for detection of SARS-CoV-2 on environmental surfaces.

Not for clinical use.

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The DirectDetect SARS-CoV-2 Real-time RT-PCR kit for Environmental Surfaces offers an efficient workflow eliminating the need for an extraction and heat lysis step. The simplified process can move from sample directly to PCR giving a broader range of customers the ability to perform environmental surface tests. The DirectDetect SARS-CoV-2 Direct Real-time RT-PCR kit assay uses reverse transcription of SARS-CoV-2 RNA, DNA amplification, and fluorescence detection to determine if a surface is contaminated with SARS-CoV-2 targeting specific genomic regions of SARS-CoV-2: nucleocapsid (N) gene and open reading frame 1ab (ORF1ab) gene. This kit includes 96 tests.


Automation Compatible Yes
Quantity 96.0
Research Areas Service Labs, Food
Sample Type Environmental Swab
Shipping Condition Dry Ice
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Application Brief

Detection of SARS-CoV-2 in Wastewater

Due to it’s serious impact on world health, society and the economy, understanding the extent of the COVID-19 pandemic is an ongoing key challenge for public health officials, even in consideration of the vaccine roll out.

Wastewater monitoring, also known as wastewater-based epidemiology (WBE), continues to play a crucial part for early identification of SARS-CoV-2 infection rates and hot spots and the surveillance of COVID-19. The demand for highly sensitive, mass-scale, cost effective and non-invasive solutions for the surveillance of the infection continues to be great.

In this Application Brief, global genomics sequencing laboratory, Dante Labs, has demonstrated how the identification of the SARS-CoV-2 virus within wastewater samples is achievable through both manual and automated workflows. The automated workflow that Dante Labs utilized includes RNA isolation sample prep with the use of the chemagic Viral DNA/RNA kit special H96 with the chemagic 360 instrument for RNA extraction, the JANUS® G3 PCR Workstation for automated SARS-CoV-2 PCR assay setup and SARS-COV-2 RT-PCR kit from PerkinElmer.

PDF 596 KB


Surface Testing for SARS-COV-2 Detection

PerkinElmer enables customers to detect SARS-CoV-2 on environmental surfaces using proven PerkinElmer technology. Streamline surface testing of SARS-Cov-2 with an automated workflow using the PerkinElmer® SARS-CoV-2 Nucleic Acid Detection Kit for Environmental Surfaces or take a simplified, direct approach by moving from sample to PCR with the DirectDetect SARS-CoV-2 Real-time RT-PCR kit for Environmental Surfaces.