The DELFIA Eu-N1 ITC chelate is optimized for the europium labeling of proteins and peptides for use in dissociation-enhanced time-resolved fluorometric assays. Isothiocyanate (ITC) activated chelates react primarily with free amine groups such as those at the N-terminus of protein or lysine residues.
Each kit contains 0.4 mg labeling reagent plus Eu-standard, DELFIA Enhancement Solution, Eu-stabilizer, purified BSA for increasing the stability of labeled proteins, an uncoated microtitration strip plate, DELFIA Assay Buffer and DELFIA Wash Concentrate. The kit contents are sufficient for a labeling of up to 1 mg of protein or antibody.
| Automation Compatible | Yes |
|---|---|
| Detection Method | Time-Resolved Fluorescence (TRF), DELFIA TRF |
| Experimental Type | In vitro |
| Fluorophore | Eu-N1-ITC |
| Product Brand Name | DELFIA |
| Quantity in a Package Amount | 0.4 mg |
| Shipping Condition | Blue Ice |
| Unit Size | 1 Kit |
The DELFIA® system provides a method of discovering specific, high-affinity monoclonal antibodies (Mabs) even in low concentrations in hybridoma supernatants. The assay used in these, conditions has to be very sensitive, specific, reliable, reproducible and easy enough to allow handling of about 1,000 to 1,500 samples in one batch.
DELFIA Eu-labeling Reagent is optimized for the europium labeling of proteins and peptides.
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