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DELFIA Assay Buffer is a buffered protein and detergent solution intended for use in DELFIA assays that include lanthanide-labelled proteins. It is optimized to give minimal fluorescence background and non-specific binding in solid phase DELFIA time-resolved fluorescence (TRF) assays. The formulation includes ready-to-use Tris-HCl buffered NaCl solution (pH 7.8) containing 0.05% NaN3, bovine serum albumin (BSA), bovine gamma globulins, Tween 40, diethylenetriaminpentaacetic acid (DTPA) and an inert red dye.
|Detection Method||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA|
|Unit Size||250 mL|
The availability of monoclonal antibodies and the almost simultaneous progress in several non-isotopic labelling techniques have contributed to recent improvements in immunochemical assays.
DELFIA is a heterogeneous time-resolved fluorometric assay method in which an enhancement step assures high sensitivity andwide response range. To achieve the best results in a DELFIA assay, the optimal buffer composition should be chosen. A numberof ready-to-use buffer products are available as catalog items, or users may prepare their own formulations based on the following guidelines.