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DELFIA Assay Buffer is a buffered protein and detergent solution intended for use in DELFIA assays that include lanthanide-labelled proteins. It is optimized to give minimal fluorescence background and non-specific binding in solid phase DELFIA time-resolved fluorescence (TRF) assays. The formulation includes ready-to-use Tris-HCl buffered NaCl solution (pH 7.8) containing 0.05% NaN3, bovine serum albumin (BSA), bovine gamma globulins, Tween 40, diethylenetriaminpentaacetic acid (DTPA) and an inert red dye.
|Detection Method||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA|
|Unit Size||250 mL|
DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs), especially at the low concentrations that may be present in hybridoma supernatants. An assay used in these conditions must be sensitive, specific, reliable, reproducible and easy enough to handle 1,000 to 1,500 samples in one batch.
The availability of monoclonal antibodies and the almost simultaneous progress in several non-isotopic labelling techniques have contributed to recent improvements in immunochemical assays.
DELFIA is a heterogeneous time-resolved fluorometric assay method in which an enhancement step assures high sensitivity andwide response range. To achieve the best results in a DELFIA assay, the optimal buffer composition should be chosen. A numberof ready-to-use buffer products are available as catalog items, or users may prepare their own formulations based on the following guidelines.