DELFIA® Toolbox kits allow you to build your own DELFIA time-resolved fluorescence (TRF) assay using your preferred antibody against the analyte of interest. Painlessly switch your traditional ELISA to one with a wider dynamic range, higher sensitivity, and great signal stability. This kit contains all necessary reagents to perform a sandwiching immunoassay using DELFIA Europium-labeled anti-rabbit IgG antibody for detection.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Build your own assay for quantitative detection of target analyte in cell lysate, cell supernatant, tissue extract, serum, plasma, blood, and other sample types using DELFIA time-resolved fluorescence wash-based technology. DELFIA (Dissociation-enhanced lanthanide fluorescence immunoassay) TRF (time-resolved fluorescence) technology is a highly-sensitive wash-based immunoassay platform which utilizes a lanthanide chelate to reduce background and improve assay dynamic range. DELFIA offers significant advantages over traditional ELISA, while keeping a similar workflow:
DELFIA Toolbox Kits allow you to build your own DELFIA assay using your preferred antibody against the analyte of interest, bringing you the ability to painlessly switch your traditional ELISA to one with a wider dynamic range, higher sensitivity and great signal stability. These kits contain:
|Assay Technology||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Detection Method||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA|
|Shipping Condition||Blue Ice|
|Unit Size||1 ½AreaPlate|
DELFIA® (Dissociated-Enhanced Lanthanide Fluorescent Immunoassay) TRF is a proven, robust immunodetection platform with over 20 years of history. DELFIA has a similar assay principle and workflow to that of a traditional ELISA, but with the added benefits of a stable, time-resolved fluorescent signal, and improved assay dynamic range.
Download this application note to see how DELFIA TRF outperforms ELISA as a robust assay providing high sensitivity, superior signal stability, and a wider dynamic range.
Along with the expression of the protein of interest, host cells release additional proteins (Host Cell Proteins, HCPs) into the supernatant which must be removed from drug products because they pose a risk of affecting drug quality, safety and efficacy.
The potential immunogenicity of biologics due to HCP contamination is a large risk, hence the need for accurate measurement to track the reduction of HCPs throughout the manufacturing process.
In this application note, you will learn:
Find out about our range of integrated solutions for drug discovery screening in this e-brochure.
Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automated liquid handling, assays and reagents, imaging and detection systems, and informatics.
Working independently or together, with our solutions you can achieve consistent and accurate results. By accelerating the identification and characterization of effective and safe drug candidates, the PerkinElmer portfolio enables you to optimize efficiency in your lab and deliver more actionable, real-world results.
Download the brochure to learn more about how we can partner with you so that you can discover smarter, more effective, data-driven breakthroughs in the critical screening stages of drug discovery and development.