DELFIA® Toolbox kits allow you to build your own DELFIA time-resolved fluorescence (TRF) assay using your preferred antibody against the analyte of interest. Painlessly switch your traditional ELISA to one with a wider dynamic range, higher sensitivity, and great signal stability. This kit contains all necessary reagents to perform a sandwiching immunoassay using DELFIA Europium-labeled anti-goat IgG antibody for detection.
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Build your own assay for quantitative detection of target analyte in cell lysate, cell supernatant, tissue extract, serum, plasma, blood, and other sample types using DELFIA time-resolved fluorescence wash-based technology. DELFIA (Dissociation-enhanced lanthanide fluorescence immunoassay) TRF (time-resolved fluorescence) technology is a highly-sensitive wash-based immunoassay platform which utilizes a lanthanide chelate to reduce background and improve assay dynamic range. DELFIA offers significant advantages over traditional ELISA, while keeping a similar workflow:
DELFIA Toolbox Kits allow you to build your own DELFIA assay using your preferred antibody against the analyte of interest, bringing you the ability to painlessly switch your traditional ELISA to one with a wider dynamic range, higher sensitivity and great signal stability. These kits contain:
|Assay Technology||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Detection Method||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA TRF|
|Shipping Condition||Blue Ice|
|Unit Size||5 stripwell plates|
DELFIA® (Dissociated-Enhanced Lanthanide Fluorescent Immunoassay) TRF is a proven, robust immunodetection platform with over 20 years of history. DELFIA has a similar assay principle and workflow to that of a traditional ELISA, but with the added benefits of a stable, time-resolved fluorescent signal, and improved assay dynamic range.
Download this application note to see how DELFIA TRF outperforms ELISA as a robust assay providing high sensitivity, superior signal stability, and a wider dynamic range.
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