Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
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This Cyanine 3 labeled analog of cytidine triphosphate (CTP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. Cyanine 3 labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. Labeled products may be detected directly via fluorescence.
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Fluorescent nucleotide analogs are biologically active with a variety of DNA polymerases. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling.