A microporous polyvinylidene difluoride membrane, PolyScreen is an effective alternative to nitrocellulose membranes for protein transfers.
- Sensitive: detects low levels of protein and decreases exposure times
- Easy to use: adapts to most nitrocellulose membrane protocols with the addition of a pre-wetting step
- Durable: stronger than nitrocellulose, it won’t tear, crack or shrink, so there are no rips or crackles on your autorads and your molecular weight markers are not distorted
- Reprobable: retains more protein than nitrocellulose, even during the most stringent washes
- Flexible: for use with standard blocking reagents (nonfat dry milk, casein, non-ionic surfactants or BSA) and all common immunostains, chromogenic and chemiluminescent substrates
- Wettable: entire membrane is prepared consistently, so transfers are more efficient
- Archivable: won’t shatter when dry, or discolor over time
- Safe: nonflammable, requires no special handling, storage or shipping precautions
- Versatile: can be used for protein staining (compatible with commonly used stains and withstands high solvent concentrations in rapid destaining), immunoblotting, protein sequencing and nitrosylation studies
