This product contains living cells. Some AequoZen™ FroZen Cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
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FroZen Cells for Calcium Testing: Chemotactic Peptide, C5a, Human Recombinant, CHO-K1. We provide one vial of frozen cells (10 million cells/vial). Some of our Frozen cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information. You are authorized to utilize these frozen cell preparations one time only. Any attempt to transfer, re-use, or propagate these cells is expressly unauthorized and a violation of the product terms and conditions of sale.
The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. That means that we will culture the cells and freeze them with an optimized protocol to stop all replication. These cells are then stored in liquid nitrogen or simply in a -80°C freezer, thawed and used directly in a functional, cellular GPCR test with previously validated performance.
|Assay Target Class||GPCR|
|Assay Target Type||Cell line|
|Assay Validated||Calcium Luminescence|
|G-Alpha Natural Receptor||Gi/Go|
|Product Brand Name||AequoZen|
|Second Messenger Release||Calcium flux|
|Shipping Condition||Dry Ice|
|Unit Size||10 million cells|
Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.
Life science researchers are always working toward the next breakthrough discovery. For scientists working on GPCRs, speed is everything. And when it comes to drug discovery, overcoming the challenge of decreasing assay development time and accelerating your research, is necessary for success.
Come explore one of the largest GPCR cell lines portfolio with more than:
PerkinElmer’s validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. So you can concentrate on results.