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Chemokine CXCR3 AequoScreen cell line

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Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.

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This product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.

ES-142-A

18500.00 USD

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Overview
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Overview

AequoScreen^® Double Transfected Cell Lines: CXCR3. Human Recombinant, in CHO-K1 host cell. Two vials of cryopreserved cells are shipped per order. A detailed technical dossier includes sequence, culture conditions and pharmacological properties of the recombinant receptor. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some products are not available in some countries. Please inquire at your local sales office for more information.

Features:

Fully automatable and miniaturizable to 1536
Enables reduction in reagents and consumable costs
High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
Robust assay (Z', CV values, solvent resistance)
Reduces the number of false positives from autofluorescent compounds in screening libraries
Option to work with cells in suspension to increase throughput
Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

Specifications

Assay Target Class	GPCR
Assay Target Type	Cell line
Assay Validated	Calcium Luminescence, Calcium Fluorescence
G-Alpha Natural Receptor	Gi/Go
Host Cell	CHO-K1
Product Brand Name	AequoScreen
Receptor Type	Chemokine
Shipping Condition	Dry Ice
Therapeutic Area	Inflammation
Unit Size	5 million cells

Resources, Events & More

Brochure

Take the Stress Out of Functional Testing - AequoZen and cAMPZen ready-to-use cells

PerkinElmer’s validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. So you can concentrate on results.

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Manual

AequoZen Frozen Cells Starter Kit

G protein coupled receptors (GPCRs) have been considered as a highly “druggable” target for many years, with over 40% of marketed drugs acting to modulate their function. For many years, radiometric techniques have dominated GPCR screening. However in the last decade the development of functional assays, where the effect of molecules is evaluated in terms of GPCR activation, has accelerated.In particular, measurement of calcium signaling and the development of molecular strategies which couple the majority of GPCRs to calcium signaling has allowed the use of high-throughput functional screening in GPCR research.

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Tools