PerkinElmer
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Chemokine CCR8 receptor (human recombinant) AequoZen Frozen Cells

Our frozen, irradiated AequoZen cells express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.

For research use only. Not for use in diagnostic procedures.

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ES-136-AF
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Overview

AequoZen® Double Transfected Cell Lines: CCR8 receptor, human recombinant, in CHO-K1 host cell. One vial of non-propagatable cells is shipped per order. A detailed technical dossier includes sequence, culture conditions and pharmacological properties of the recombinant receptor. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some products are not available in some countries. Please inquire at your local sales office for more information.

Features:

  • Ready-to-use cells; no cell culture required (dispense and assay)
  • Fully automatable and miniaturizable to 1536
  • Enables reduction in reagents and consumable costs
  • High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
  • Robust assay (Z', CV values, solvent resistance)
  • Reduces the number of false positives from autofluorescent compounds in screening libraries
  • Option to work with cells in suspension to increase throughput
  • Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

AequoZen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

Specifications

Assay Target Class GPCR
Assay Validated Calcium Luminescence
Automation Compatible Yes
Host Cell CHO-K1
Product Brand Name AequoZen
Receptor Type Chemokine
Shipping Condition Dry Ice
Therapeutic Area Inflammation
Unit Size 10 million cells
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Brochure

Take the Stress Out of Functional Testing - AequoZen and cAMPZen ready-to-use cells

PerkinElmer’s validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. So you can concentrate on results.

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Guide

GDE_Radioligand_selector_guide

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Manual

AequoZen Frozen Cells Starter Kit

G protein coupled receptors (GPCRs) have been considered as a highly “druggable” target for many years, with over 40% of marketed drugs acting to modulate their function. For many years, radiometric techniques have dominated GPCR screening. However in the last decade the development of functional assays, where the effect of molecules is evaluated in terms of GPCR activation, has accelerated.In particular, measurement of calcium signaling and the development of molecular strategies which couple the majority of GPCRs to calcium signaling has allowed the use of high-throughput functional screening in GPCR research.

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