PerkinElmer
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chemagic cfDNA 5k Kit H24

The chemagic cfDNA 5k Kit H24 can be used for automated cfDNA isolation from 3 - 5 ml plasma or serum samples on the chemagic 360 instrument equipped with the chemagic 24 Rod Head. The chemagic kits include all required reagents and disposable plastic ware for nucleic acid purification.

For research use only. Not for use in diagnostic procedures.

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CMG-1304
5600.00 USD
 
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Overview

In the event of apoptosis, necrosis or break-down of pathogens, or cells, such as white blood cells circulating cell-free DNA (cfDNA) is floating through the blood. As the average size of cfDNA is 170 bp (50 – 200 bp) and only 1 – 50 ng of cfDNA/ml of plasma is present, it is quite difficult to separate this hay in the haystack. Nevertheless the analysis of cfDNA in blood is an elegant non-invasive method to study cancer or chromosomal disorders. PerkinElmer chemagen has a new method to isolate cfDNA form 0.5 - 5 ml plasma with minimal hands-on time with the chemagic 360 instrument.

Product Specifications

The kit is designed for the use with plasma samples derived from EDTA, citrate or other collection tubes. Fresh and frozen plasma can be used.

Technology

The chemagic cfDNA Kits are based on chemagic Technology using M-PVA Magnetic Beads for the isolation of cfDNA. The cfDNA binds to paramagnetic beads which are magnetically separated from the sample material. During subsequent steps contaminants are removed and the purified cfDNA is transferred into an elution medium. The automated sample processing by the chemagic 360 instrument excludes cross contamination and ensures safe handling of infectious sample material.

Plasma Preparation Protocol

It is recommended to prepare plasma as fresh as possible (max. 5 days after blood draw). Longer storage of blood prior to plasma preparation may lead to poor separation results and higher background from genomic DNA.

A double centrifugation protocol during plasma preparation is recommended to minimize the potential for contamination of plasma with cells and genomic DNA. Transfer of any other blood components (buffy coat or red blood cells) should be avoided while separating the plasma fraction.

From 10 ml of whole blood approximately 4 - 5 ml plasma can be expected.

  • Centrifuge whole blood collection tube at 2000 x g for 20 minutes.
  • Aspirate plasma carefully and at least 2 - 3 mm above the buffy coat layer, without disturbing the layer and transfer it into a new appropriate tube.
  • Centrifuge the plasma sample at 3300 x g for 30 minutes.
  • Carefully transfer the supernatant to a fresh tube without disturbing the pellet, at least 2 - 3 mm above the pellet.
  • For direct use storage of plasma sample at 2 - 8 °C is possible, for long-term storage -80 °C is recommended.

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Features

Model Compatible Withchemagic 360 instrument

Specifications

Sample Type Plasma, Serum
Sample Volume 3 - 5 mL
Unit Size 240 Preps/Kit