For research use only. Not for use in diagnostic procedures.
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In the event of apoptosis, necrosis or break-down of pathogens, or cells, such as white blood cells circulating cell-free DNA (cfDNA) is floating through the blood. As the average size of cfDNA is 170 bp (50 – 200 bp) and only 1 – 50 ng of cfDNA/ml of plasma is present, it is quite difficult to separate this hay in the haystack. Nevertheless the analysis of cfDNA in blood is an elegant non-invasive method to study cancer or chromosomal disorders. PerkinElmer chemagen has a new method to isolate cfDNA form 0.5 - 5 ml plasma with minimal hands-on time with the chemagic 360 instrument.
The kit is designed for the use with plasma samples derived from EDTA, citrate or other collection tubes. Fresh and frozen plasma can be used.
The chemagic cfDNA Kits are based on chemagic Technology using M-PVA Magnetic Beads for the isolation of cfDNA. The cfDNA binds to paramagnetic beads which are magnetically separated from the sample material. During subsequent steps contaminants are removed and the purified cfDNA is transferred into an elution medium. The automated sample processing by the chemagic 360 instrument excludes cross contamination and ensures safe handling of infectious sample material.
It is recommended to prepare plasma as fresh as possible (max. 5 days after blood draw). Longer storage of blood prior to plasma preparation may lead to poor separation results and higher background from genomic DNA.
A double centrifugation protocol during plasma preparation is recommended to minimize the potential for contamination of plasma with cells and genomic DNA. Transfer of any other blood components (buffy coat or red blood cells) should be avoided while separating the plasma fraction.
From 10 ml of whole blood approximately 4 - 5 ml plasma can be expected.
For more information, please click here.
|Model Compatible With||chemagic™ 360 instrument|
|Sample Type||Plasma, Serum|
|Sample Volume||3 - 5 mL|
|Unit Size||240 Preps/Kit|