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Brownlee SPP Peptide ES C18 Column - 50 mm x 4.6 mm I.D.

Sterically protected ligand provides greater stability at low pH where most peptide separations are performed.

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The Brownlee SPP (Superficially Porous Particle) Peptide ES C18 column has a 160 Å pore size, specifically chosen for the molecular weight range of peptides. The ligand was chosen due to its sterically protected bonding technology that inhibits acid hydrolysis of the siloxane bonds, even under extremes of high temperature and low pH. This column is filled with 2.7 μm superficially porous particles that contain a 1.7 μm solid inner core and a 0.5 μm porous silica outer shell, allowing for a shorter diffusion path and nearly double the efficiency compared to traditional fully porous silica particles. It can be used to enhance the performance of both traditional HPLC systems and UHPLC systems and is compatible with 2 µm inlet frits, so traditional solvents and sample preparation techniques can be used. Specialized filters are not needed for UHPLC systems.

  • Coverage: 2.0 μmol/m²
  • End-Capping: No
  • pH Range: 1.0-8.0
  • Pressure Limit (UHPLC): 9,000 psi (620 bar)
  • Temperature Limit: 30℃
  • USP Code: L1


Inner Diameter 4.6 mm
Length 50.0 mm
Particle Size 2.7 µm
Phase Peptide ES-C18
Product Group Conventional Columns
Technology Type Liquid Chromatography (LC)