Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
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This biotin labeled analog of guanosine triphosphate (GTP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. Biotin labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. Biotin-labeled products may be detected with streptavidin conjugates. For the highest sensitivity in detection, we recommend using a streptavidin-HRP conjugate with tyramide signal amplification (TSA).
The number “11” in the compound name refers to the length of the linker arm between GTP and the label.
| buffer | 10 mM Tris-HCl, pH 7.6, 1 mM EDTA |
|---|---|
| Concentration | 1 mM |
| Detection Method | Indirect |
| Hapten | Biotin |
| Nucleotide | GTP |
| Shipping Condition | Ambient |
| Unit Size | 25 nmol |
TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.