PerkinElmer
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BCIP/NBT Chromogenic Substrate (2 x 250 mL)

BCIP/NBT substrate detects alkaline phosphatase in blotting and slide applications. Available in ready to use solution, it deposits a permanent dark purple stain on membrane sites bearing phosphatase and with the combination of BCIP(5-bromo-4-chloro-3indolyl-phosphate and NBT(nitrobluetetrazoleum) produces much higher sensitivity than either reagent separately.

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NEL937001PK
171.00 USD
 
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Overview

Use enough reagent to completely cover the membrane surface or at least 0.25 ml/cm2 of membrane.

This substrate solution is formulated for use on PolyScreen® PVDF Transfer Membrane, nitrocellulose membranes and the nylon membranes GeneScreen Hybridization Transfer Membrane and GeneScreen Plus® Hybridization Transfer Membrane.

Specifications

Detection Method Absorbance/Colorimetric
Quantity in a Package Amount 2.0 Units
Shipping Condition Ambient
Unit Size 250 mL