BCIP/NBT substrate detects alkaline phosphatase in blotting and slide applications. Available in ready to use solution, it deposits a permanent dark purple stain on membrane sites bearing phosphatase and with the combination of BCIP(5-bromo-4-chloro-3indolyl-phosphate and NBT(nitrobluetetrazoleum) produces much higher sensitivity than either reagent separately.
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Use enough reagent to completely cover the membrane surface or at least 0.25 ml/cm2 of membrane.
This substrate solution is formulated for use on PolyScreen® PVDF Transfer Membrane, nitrocellulose membranes and the nylon membranes GeneScreen™ Hybridization Transfer Membrane and GeneScreen Plus® Hybridization Transfer Membrane.
|Quantity in a Package Amount||2.0 Units|
|Unit Size||250 mL|