BCIP/NBT substrate detects alkaline phosphatase in blotting and slide applications. Available in ready to use solution, it deposits a permanent dark purple stain on membrane sites bearing phosphatase and with the combination of BCIP(5-bromo-4-chloro-3indolyl-phosphate and NBT(nitrobluetetrazoleum) produces much higher sensitivity than either reagent separately.
true falseUse enough reagent to completely cover the membrane surface or at least 0.25 ml/cm2 of membrane.
This substrate solution is formulated for use on PolyScreen® PVDF Transfer Membrane, nitrocellulose membranes and the nylon membranes GeneScreen™ Hybridization Transfer Membrane and GeneScreen Plus® Hybridization Transfer Membrane.
| Detection Method | Absorbance/Colorimetric |
|---|---|
| Quantity in a Package Amount | 2.0 Units |
| Shipping Condition | Ambient |
| Unit Size | 250 mL |