check quantity

ATPlite Luminescence Assay System, 300 Assay Kit

ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells.

Part Number
Unit Size
List Price
Your Price
300 (96-well) / 1200 (384-well)
146.00 USD
1,000 assay points (96-well) / 4,000 (384-well)
420.00 USD
5,000 (96-well) / 20,000 (384-well)
1393.00 USD
10,000 (96-well) / 40,000 (384-well)
2786.00 USD
Buy Now

Please enter valid quantity

Please log in to add favorites.



ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects. Our ATPlite and ATPlite 1step luminescence assay systems use patented innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.

ATPlite is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence assay is the alternative to colorimetric, fluorometric and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.


  • Long-lived luminescent signal: half-life (t1/2) greater than 5 hours, depending on cell type and medium
  • Rapid: results generated in 15 - 25 minutes
  • Simple and reproducible: no separation steps; only two reagent additions
  • Homogeneous assay: no cell harvesting or centrifugation required
  • Sensitive: down to 5 cells in 100µL medium (derived from CHO and HL-60 cells in 100µL medium)
  • Wide linear dynamic range: =105 (as derived from CHO and HL-60 cells)


Assay Protocol 2-step
Automation Compatible Yes
Detection Method Luminescence
Experimental Type In vitro
Product Brand Name ATPlite
Shipping Condition Ambient
Unit Size 300 (96-well) / 1200 (384-well)
Resources, Events & More
  • All




PDF 630 KB

White Paper

Cell-Based Assays: Purposeful Screens for Better Results

Over these last few decades there has been a growing trend in drug discovery to use cellular systems and functional assays, in addition to biochemical assays, for the characterization of new potential therapeutics. The ability to study the interaction between a candidate drug and its target within the context of a whole, intact cell allows for more physiologically relevant data to be obtained. However, such assays are more complex than traditional biochemical assays as such facts as membrane permeability, cellular metabolism, cell variability, additional binding partners, and signal transduction must be considered.

To help you navigate the complexities in designing cell-based assays, we have gathered insights collected over the years and compiled them to provide you with elements to consider when setting up your cell-based assays. After all, any assay, biochemical or cell-based, is only as good as its design.