AlphaLISA® Acceptor beads conjugated to anti-V5 antibody. The mouse monoclonal antibody detects proteins tagged with the V5 14-amino-acid tag (the 9-amino-acid tag has also been successfully bound by the antibody).
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For research use only. Not for use in diagnostic procedures.
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These beads can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for:
In a typical AlphaLISA assay, 25 mg of Acceptor beads is sufficient to run 25,000-50,000 wells using a 50 µL reaction volume.
|Bead Type or Core Bead Type||AlphaLISA Acceptor|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||25 mg|
The interactions and bindingof proteins are implicated in a large number of biological processes. The needfor an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measurement of protein interactions and complexes as large as 200 nm in size
Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.
This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.
AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.