PerkinElmer
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Anti-rabbit PVT 500 MG

Anti-rabbit-coated SPA beads, for capturing rabbit IgG antibodies in proximity-based radiometric scintillation assays.

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Detail Information

SPA Scintillation beads are microspheres containing scintillant which emit light in the blue region of the visible spectrum. As a result, these beads are ideally suited to use with photomultiplier tube (PMT) counters such as the MicroBeta2 or TopCount.

Two types of core SPA Scintillation bead are available - yttrium silicate (YSi) and Polyvinyltoluene (PVT). PVT beads are plastic, larger in size, and stay in suspension longer than the crystalline YSi beads.

Scintillation proximity assay (SPA) is a homogeneous and versatile technology for the rapid and sensitive assay of a wide range of biological processes, including applications using enzyme and receptor targets, radioimmunoassays, and molecular interactions. When 3H, 14C, 33P, and 125I radioisotopes decay, they release β-particles (or Auger electrons, in the case of 125I). The distance these particles travel through an aqueous solution is dependent on the energy of the particle. If a radioactive molecule is held in close enough proximity to a SPA Scintillation Bead or a SPA Imaging Bead, the decay particles stimulate the scintillant within the bead to emit light, which is then detected in a PMT-based scintillation counter or on a CCD-based imager, respectively. However, if the radioactive molecule does not associate with the SPA bead, the decay particles will not have sufficient energy to reach the bead and no light will be emitted. This discrimination of binding by proximity means that no physical separation of bound and free radiochemical is required.

Specifications

Automation Compatible Yes
Bead Type or Core Bead Type PVT
Coating Treatment Anti-rabbit antibody, Anti-rabbit IgG
Detection Method Radiometric
Product Brand Name SPA Scintillation Beads
Shipping Condition Ambient
Unit Size 500 mg
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Application Note

Assay Development Guide to Protein:Protein Studies

Interactions between proteins are a key feature of many biochemical processes, for example cell signalling. In the absence of any enzymatic activity, measurement of protein:protein interactions has presented problems. Scintillation Proximity Assay (SPA) technology permits the direct measurement of binding of one protein to another.

PDF 951 KB
The Application of SPA Technology to Study Protein: DNA Interactions

Many proteins are able to recognize and subsequently bind DNA. These proteins were first identified by their presence in isolated DNA complexes, by their ability to bind DNA in vitro, and by their absolute requirement in many DNA-dependent functions.

PDF 997 KB
The Application of SPA to SH2 and SH3 Domain Binding to Specific Peptide Sequences

SH2 and SH3 domains are small, independent domains of about 100 or 70 amino acid residues respectively. They are foundin a variety of proteins, and can occur together or separately. SH2 domains are thought to be involved in signal transduction mechanisms. Some SH2 domains control enzyme activity by binding membrane-bound receptors to regulate downstream events such as kinase cascades.

PDF 1 MB