PerkinElmer
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Anti-6X His AlphaPlex 645 (Sm) Acceptor beads, 25 mg

AlphaPlex®-645 (Samarium) Acceptor beads conjugated to anti-6XHis antibody, for use in Alpha no-wash multiplexing assays.

This is a replacement for AP128SM.

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AP178SM-C
250 μg
824.00 USD
 
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AP178SM-M
5 mg
8400.00 USD
 
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AP178SM-R
25 mg
33800.00 USD
 
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Overview

AlphaPlex®-645 (Samarium) Acceptor beads conjugated to anti-6XHis antibody. The mouse monoclonal antibody detects proteins containing accessible consecutive histidine residues at the amino or carboxy terminus. These beads can be used to capture His-tagged proteins and peptides, and can be used with Alpha Donor beads to create AlphaPlex no-wash assays for:

  • Protein-protein interaction assays
  • Protein detection assays
  • Enzymatic assays

AlphaPlex Acceptor beads are intended to be used in multiplexing assays, in conjunction with AlphaLISA Acceptor beads and Alpha Donor beads. In a typical Alpha assay, 250 μg of Acceptor beads is sufficient to run 250-500 wells using a 50 μL reaction volume.

Specifications

Antibody Conjugates Anti-6X His
Assay Technology Alpha
Automation Compatible Yes
Bead Type or Core Bead Type AlphaPlex Samarium (645) Acceptor
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaPlex
Shipping Condition Blue Ice
Unit Size 25 mg
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Application Note

Development of Pharmacokinetic (PK) Assays for Detecting Biosimilars Targeting TNF alpha using AlphaLISA

Anti-inflammatory monoclonal antibody drugs that specifically target TNFα, such as Humira®, have been highly successful in the market. As patents expire on these top-selling drugs, effort has been placed on developing biosimilars. Biosimilars differ from small molecule generi ...

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Brochure

Guide

ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners ...

PDF 2 MB