PerkinElmer
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Anti-6X His AlphaPlex 645 (Sm) Acceptor beads, 250 μg

AlphaPlex®-645 (Samarium) Acceptor beads conjugated to anti-6XHis antibody, for use in Alpha no-wash multiplexing assays.

This is a replacement for AP128SM.

For research use only. Not for use in diagnostic procedures.

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Unit Size
List Price
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AP178SM-C
250 μg
700.00 USD
 
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AP178SM-M
5 mg
7100.00 USD
 
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AP178SM-R
25 mg
28700.00 USD
 
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Overview

AlphaPlex®-645 (Samarium) Acceptor beads conjugated to anti-6XHis antibody. The mouse monoclonal antibody detects proteins containing accessible consecutive histidine residues at the amino or carboxy terminus. These beads can be used to capture His-tagged proteins and peptides, and can be used with Alpha Donor beads to create AlphaPlex no-wash assays for:

  • Protein-protein interaction assays
  • Protein detection assays
  • Enzymatic assays

AlphaPlex Acceptor beads are intended to be used in multiplexing assays, in conjunction with AlphaLISA Acceptor beads and Alpha Donor beads. In a typical Alpha assay, 250 μg of Acceptor beads is sufficient to run 250-500 wells using a 50 μL reaction volume.

Specifications

Antibody Conjugates Anti-6X His
Assay Technology Alpha
Automation Compatible Yes
Bead Type or Core Bead Type AlphaPlex Samarium (645) Acceptor
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaPlex
Shipping Condition Blue Ice
Unit Size 250 μg
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Brochure

Guide

ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

PDF 3 MB