Determine early stages of apoptosis with Annexin-Vivo 750.
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For laboratory use only. This product is intended for animal research only and not for use in humans.
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Annexin A5 (Annexin V) is a cellular protein in the annexin group. Annexin V is a probe commonly used to detect apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis when it is on the outer leaflet of the plasma membrane.
Annexin-Vivo™ 750, from PerkinElmer, is a targeted fluorescent imaging agent comprising this selective protein, Annexin A5 and a near-infrared (NIR) fluorochrome. This agent has been developed to enable in vivo visualization and quantification of the membrane-bound phospholipid, phosphotidylserine, exposed in the outer leaflet of the cell membrane lipid bilayer during the early stages of apoptosis.
|Fluorescent Agent Type||Targeted|
|Optical Imaging Classification||Fluorescence Imaging|
|Quantity in a Package Amount||1.0 Units|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Atherosclerosis, Oncology/Cancer|
|Unit Size||1 Vial (10 doses)|
|Wave Length||750 nm|
Drug induced liver injury (DILI) is a major reason,for late stage termination of drug discovery,research projects, highlighting the importance,of early integration of liver safety assessment in the drug development process. A technical,approach for in vivo toxicology determination was developed using Acetaminophen (APAP),a commonly used over-the-counter analgesic and antipyretic drug, to induce acute,hepatocellular liver injury. PerkinElmer imaging technology and near infrared (NIR) labeled,Annexin V (Annexin-VivoTM 750) were used to detect and quantify and necrosis associated,with this type of liver toxicity. Both fluorescent tomographic imaging (FMT® 4000 and IVIS®,SpectrumCT), and higher throughput epifluorescence imaging (IVIS SpectrumCT), provided,excellent detection of Annexin-Vivo fluorescence in the liver. Histology and plasma alanine,transaminase (ALT) confirmed the kinetics of tissue necrosis, and more extensive liver damage,was seen but with an apparent decrease in tissue PS and plasma ALT by 48 hours, suggesting,a decline in the induction of tissue destruction. Compared to conventional plasma/serum,assays, in vivo imaging can offer fast, quantitative imaging results directly assessing the tissue,of interest.
Protocol for Annexin-Vivo 750 In Vivo Fluorescent Imaging Agent
Annexin-Vivo™ 750 is a targeted fluorescence imaging agent comprising a selective protein, Annexin A5 and a near-infrared (NIR) fluorochrome. This agent has been developed to enable in vivo visualization and quantification of the membrane-bound phospholipid, phosphotidylserine, exposed in the outer leaflet of the cell membrane lipid bilayer during the early stages of apoptosis.