AlphaScreen Glutathione Donor beads 25 mg

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

The interactions and bindingof proteins are implicated in a large number of biological processes. The needfor an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measurement of protein interactions and complexes as large as 200 nm in size

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

Mitogen-activated protein kinases, MAPKs, are constituents of numerous signal transduction pathways, and are activated by protein kinase cascades. There is great interest in finding compounds that target components of the MAPK pathways as possible treatment for inflammatory diseases and cancer. Here we report the development of a novel AlphaScreen® assay to measure MEK2 mediated phosphorylation of Erk-1.

Protein kinases are directly implicated in many human diseases, including inflammatory diseases and cancer; thus, kinases are promising therapeutic targets. In kinase screening and deorphanization studies, the use of protein substrates constitutes a major advantage over peptide substrates. The AlphaScreen® PhosphoSensor™ GST kit was developed to simplify kinase assays using physiological substrates.