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AlphaScreen no-wash assay kit for quantitative detection of cAMP. In this AlphaScreen competition assay, a biotinylated cAMP tracer binds to Streptavidin-coated Donor beads, and interacts with anti-cAMP antibody conjugated to AlphaScreen Acceptor beads. When no cAMP is present, the beads come into close proximity. The excitation of the Donor beads triggers the release of singlet oxygen molecules, which diffuse to the Acceptor beads, resulting in light emission at 520-620 nm. With increasing concentrations of endogenous cAMP, fewer Donor and Acceptor beads are brought together, resulting in a decrease in signal.
This kit contains enough reagents to run 1,000 wells using a 25 µL reaction volume. Protocol can be found on the Certificate of Analysis.
This product replaces 6760625R.
|Product Brand Name||AlphaScreen|
|Quantity in a Package Amount||1.0 per pack|
|Second Messenger Release||cAMP|
|Shipping Condition||Blue Ice|
|Unit Size||50,000 assay points|