PerkinElmer
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Anti-DIG Alpha Donor beads, 5 mg

Alpha Donor beads conjugated to anti-DIG (digoxin) antibody. This bead can be used to capture Dig-labeled biomolecules for Alpha no-wash assays.

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For research use only. Not for use in diagnostic procedures.

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Unit Size
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AS108D
1 mg
514.00 USD
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AS108M
5 mg
2009.00 USD
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AS108R
25 mg
8700.00 USD
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Detail Information

Alpha Donor beads conjugated to anti-Digoxin antibody. Anti-Dig beads are a good alternative to streptavidin Donor beads for assays where biotin in samples (e.g., culture media) might interfere. These beads can be used to capture Dig-labeled antibodies, proteins and peptides, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

  • Analyte detection assays
  • Protein-protein interaction assays
  • Protein-DNA interaction assays
  • Protein-RNA interaction assays
  • Protein-small molecule interaction assays
  • Protein detection assays
  • Enzymatic assays

In a typical Alpha assay, 1 mg of beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

Specifications

Antibody Conjugates Anti-DIG
Automation Compatible Yes
Bead Type or Core Bead Type Alpha Donor
Product Brand Name Alpha
Quantity in a Package Amount 1.0 Units
Shipping Condition Blue Ice
Unit Size 5 mg
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Brochure

Guide

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

PDF 380 KB
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

PDF 3 MB