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Anti-DIG Alpha Donor beads, 25 mg

Alpha Donor beads conjugated to anti-DIG (digoxin) antibody. This bead can be used to capture Dig-labeled biomolecules for Alpha no-wash assays.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
Quantity
AS108D
1 mg
721.00 USD
 
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AS108M
5 mg
2817.00 USD
 
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AS108R
25 mg
12300.00 USD
 
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Overview

Alpha Donor beads conjugated to anti-Digoxin antibody. Anti-Dig beads are a good alternative to streptavidin Donor beads for assays where biotin in samples (e.g., culture media) might interfere. These beads can be used to capture Dig-labeled antibodies, proteins and peptides, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

  • Analyte detection assays
  • Protein-protein interaction assays
  • Protein-DNA interaction assays
  • Protein-RNA interaction assays
  • Protein-small molecule interaction assays
  • Protein detection assays
  • Enzymatic assays

In a typical Alpha assay, 1 mg of beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

Specifications

Antibody Conjugates Anti-DIG
Automation Compatible Yes
Bead Type or Core Bead Type Alpha Donor
Product Brand Name AlphaLISA
Quantity in a Package Amount 1.0 Units
Shipping Condition Blue Ice
Unit Size 25 mg
Resources, Events & More
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Application Note

Development of Pharmacokinetic (PK) Assays for Detecting Biosimilars Targeting TNF alpha using AlphaLISA

Anti-inflammatory monoclonal antibody drugs that specifically target TNFα, such as Humira®, have been highly successful in the market. As patents expire on these top-selling drugs, effort has been placed on developing biosimilars. Biosimilars differ from small molecule generi ...

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Brochure

Guide

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored ...

PDF 380 KB
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners ...

PDF 2 MB

Technical Note

Development of an AlphaLISA Assay to Measure and Screen Inhibitors of the p53-MDM2 Interaction

Binding events between biomolecules are important components of biological processes and a number of these biomolecular interactions have been targeted for the development of novel therapeutic drugs. p53 is a transcription factor and tumor suppressor protein that is activated in response to cellu ...

PDF 1 MB