AlphaPlex™ 645 (Samarium) TruHits kit for use as a control for interference in Alpha no-wash multiplexing assays. This kit provides enough reagents to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume.
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For research use only. Not for use in diagnostic procedures.
The AlphaPlex™ 645 (Samarium) TruHits kit allows you to perform ratiometric Alpha signal measurement with almost any type of AlphaLISA assay. This kit includes AlphaPlex 5=645 biotinylated Acceptor beads and Streptavidin Alpha Donor beads capable of interacting together to generate a signal measurable at 645 nm. In a duplex assay setup, Donor bead excitation triggers a simultaneous cascade of energy transfer within both AlphaLISA (emission = 615 nm) and AlphaPlex 645 (emission = 645 nm) Acceptor beads, leading to distinct signal emissions discriminable using either narrow bandpass filers or a dedicated double dichroic mirror. Further to facilitating the detection of non-specific interactions, the AlphaPlex TruHits kit also allows you to identify inner filters, light scatterers (insoluble compounds), singlet oxygen quenchers and biotin mimetics interfering with the Alpha signal, aiding the identification of false positives.
AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.
| Automation Compatible | Yes |
|---|---|
| Detection Method | Alpha |
| Product Brand Name | AlphaPlex |
| Shipping Condition | Blue Ice |
| Unit Size | 500 Assay Points |
