PerkinElmer
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AlphaPlex-645 (Sm) Human ICAM-1 Detection Kit, 100 Assay Points

The AlphaPlex™-645 (Sm) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
AP282SM-HV
100 Assay Points
672.00 USD
 
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AP282SM-C
500 Assay Points
3037.00 USD
 
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AP282SM-F
5,000 Assay Points
23700.00 USD
 
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Overview

The AlphaPlex™-645 (Sm) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).


Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative multiplexing assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 645 nm.

AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.

Specifications

Assay Target ICAM-1
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaPlex
Shipping Condition Blue Ice
Therapeutic Area Cardiovascular
Unit Size 100 Assay Points