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The AlphaPlex™ 645 (Samarium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
AlphaPlex technology allows the detection of molecules of interest in a no- wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 645 nm.
AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaPlex|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
This application note describes how AlphaPlex reagents from PerkinElmer can be used to simultaneously detect IL-6 and IL-8 secretion by cell lines.