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AlphaPlex-545 Human IL1β Detection Kit, 500 assay points

The AlphaPlex™ 545 Human IL1β Detection Kit is designed for the quantitative determination of human IL1β together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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Unit Size
List Price
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100 assay points
736.00 USD
500 assay points
1904.00 USD
5,000 assay points
12600.00 USD
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The AlphaPlex™ 545 Human IL1ß Detection Kit is designed for the quantitative determination of human IL1ß together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.

This kit contains reagents to run 500 assays, using a 50 µL reaction volume (5 µL of sample). Protocol can be found on the Certificate of Analysis.

AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 545 nm.

AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.


Assay Target IL1β
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaPlex
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 500 assay points
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Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.

Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.


Application Note

Simultaneous Detection of IL-6 and IL-8 Secretion by Cell Lines using AlphaPlex Technology

This application note describes how AlphaPlex reagents from PerkinElmer can be used to simultaneously detect IL-6 and IL-8 secretion by cell lines.

PDF 437 KB


Technical Note

Homogeneous Duplexing Assay for Insulin and Glucagon Quantitation Using Alpha Technology

The balance of glucagon and insulin levels within the body is an important indicator of the function of metabolism. Healthy metabolic function is characterized by elevated levels of one of these hormones and lowered levels of the other. Simultaneous high levels generally indicate hyperglycemia or type 2 diabetes, while simultaneous low levels indicate type 1 diabetes or starvation.

When testing for metabolic diseases it is important to obtain information on the level of glucagon and insulin relative to each other. Ideally this is done by quantifying the levels of both hormones from a single sample. AlphaPlex utilizes a homogenous bead-based platform for multiplexing, allowing for the quantification of multiple analytes simultaneously.

See how AlphaPlex offers a complete assay for all-in-one-well measurement of both insulin and glucagon in biological samples.