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The AlphaPlex™ 545 Human IL1ß Detection Kit is designed for the quantitative determination of human IL1ß together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
This kit contains reagents to run 500 assays, using a 50 µL reaction volume (5 µL of sample). Protocol can be found on the Certificate of Analysis.
AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 545 nm.
AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.
|Assay Target Class||Cytokine|
|Product Brand Name||AlphaPlex|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
This application note describes how AlphaPlex reagents from PerkinElmer can be used to simultaneously detect IL-6 and IL-8 secretion by cell lines.