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Anti-unmodified H3K4 AlphaPlex-545 Acceptor Beads, 25 mg

AlphaPlex-545 (Terbium) Acceptor beads conjugated to antibody against human Histone H3 that is unmodified (H3K4), for use in Alpha no-wash multiplexing epigenetic assays.

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For research use only. Not for use in diagnostic procedures.

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AP119TB-C
250 µg
1421.00 USD
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AP119TB-M
5 mg
7300.00 USD
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AP119TB-R
25 mg
25800.00 USD
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Detail Information

AlphaPlex-545 (Terbium) Acceptor beads conjugated to antibody against human Histone H3 that is unmodified (H3K4), for use in Alpha no-wash multiplexing epigenetic assays.

Alpha technology is a no-wash homogeneous proximity immunoassay that includes Alpha Donor and AlphaPlex-545 Acceptor beads to quantitate activity on histone, peptide, or protein substrates. In vitro modification of substrate is performed by incubating enzyme with appropriate cofactor and histone substrate. Following the enzymatic reaction, methylation or acetylation is monitored using specific antibody-conjugated AlphaPlex Acceptor beads. Following enzymatic reaction,streptavidin-coated Donor beads are added to capture the substrate, the beads are brought into close proximity in the absence of epigenetic mark on Histone H3 (Lysine 4) on the target substrate. The Donor beads are excited by a laser at 680 nm to release singlet oxygen. The singlet oxygen causes a reaction in the Acceptor bead and light emission is produced at 545 nm. This emission is proportional to the amount of unmodified / non-methylated / non-acetylated substrate present.

AlphaPlex-545 Acceptor beads are intended for use in multiplexing Alpha assays, in conjunction with Alpha Donor beads and AlphaLISA® Acceptor beads.

  • 250 µg of Acceptor beads is sufficient to run ~500 wells using a 50 µL reaction volume.
  • 5 mg of Acceptor beads is sufficient to run ~10,000 wells using a 50 µL reaction volume.
  • 25 mg of Acceptor beads is sufficient to run ~25,000-50,000 wells using a 50 µL reaction volume.

Specifications

Antibody Conjugates Anti-H3K4
Automation Compatible Yes
Bead Type or Core Bead Type AlphaPlex Terbium (545) Acceptor
Detection Method Alpha
Molecular Modification Unmodified
Product Brand Name AlphaPlex
Shipping Condition Blue Ice
Unit Size 25 mg
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Brochure

Guide

ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB